性猛交XXXX乱大交派对,四虎影视WWW在线观看免费 ,137最大但人文艺术摄影,联系附近成熟妇女

產(chǎn)品展廳收藏該商鋪

您好 登錄 注冊(cè)

當(dāng)前位置:
上海恒遠(yuǎn)生物科技有限公司>技術(shù)文章>恒遠(yuǎn)產(chǎn)品文獻(xiàn):生物素標(biāo)記的山羊抗兔抗體引用文獻(xiàn)

技術(shù)文章

恒遠(yuǎn)產(chǎn)品文獻(xiàn):生物素標(biāo)記的山羊抗兔抗體引用文獻(xiàn)

閱讀:692          發(fā)布時(shí)間:2021-1-11

【文獻(xiàn)標(biāo)題】Circular RNA-hsa-circ-0000670 promotes gastric cancer progression through the microRNA-384/SIX4 axis

【作者】Pengliang Liu, Shuang Cai, Nuo Li

【作者單位】中國(guó)醫(yī)科大學(xué)附屬第四醫(yī)院(the Fourth Affiliated Hospital of China Medical University)

【文獻(xiàn)中引用產(chǎn)品】

生物素標(biāo)記的山羊抗兔抗體

【關(guān)鍵詞】Hsa-circ-0000670,MicroRNA-384,Sine oculis-related homeobox 4,Gastric cancer

【DOI】doi.org/10.1016/j.yexcr.2020.112141

【影響因子(IF)】3.21

出版期刊《Experimental Cell Research》

【產(chǎn)品原文引用】

The tissues were fixed with 4% paraformaldehyde for 24 h, hydrated with 80%, 90%, 100% ethanol and n-butanol, embedded with paraffin, and cut into 5-μm-thick sections. After being heated at 60°C for 1 h, the sections were dewaxed with xylene, dehydrated with gradient ethanol (80%, 90%, 100%) and immersed in 3% H2O2 for 10 min. Antigen retrieval was performed, and the sections were then blocked with 100 μL 5% bovine serum albumin at 37°C for 30 min. Subsequently, the sections were incubated with 100 μL primary rabbit antibodies against SIX4 (1: 2000, ab176713), VEGF (1: 250, ab32152), and CD31 (1: 50, ab28364) overnight at 4°C followed by incubation with a secondary biotin-labeled goat anti-rabbit antibody (HY90046, Shanghai HengYuan Biological Co., Ltd., Shanghai, China) at 37°C for 30 min. Finally, the sections were stained with DAB chromogen (Beijing Bioss Biotechnology Co., Ltd., Beijing, China), and the cell nucleus was stained with hematoxylin for 5 min. The positive cells were stained in brown-yellow. Sections were visualized by Image-Proplus image analysis software (Media Cybernetics, Silver Springs, MD, USA). The target protein expression was evaluated by the proportions of positive cells in total cells detected from 5 randomly selected fields of view on every slide (200-fold magnification). Proportion scores were assigned as follows: positive tumor cells/total tumor cells > 10% positive (+), positive cells < 10% negative (-). 

完整版PDF文獻(xiàn)請(qǐng)咨詢(xún)?cè)诰€(xiàn)客服或者聯(lián)系我司業(yè)務(wù)員!

更多公司福利請(qǐng)關(guān)注“恒遠(yuǎn)生物”VX公眾號(hào)!

收藏該商鋪

請(qǐng) 登錄 后再收藏

提示

您的留言已提交成功!我們將在第一時(shí)間回復(fù)您~

對(duì)比框

產(chǎn)品對(duì)比 產(chǎn)品對(duì)比 聯(lián)系電話(huà) 二維碼 意見(jiàn)反饋 在線(xiàn)交流

掃一掃訪(fǎng)問(wèn)手機(jī)商鋪
13636351217
在線(xiàn)留言
弥勒县| 九龙城区| 罗源县| 凭祥市| 永登县| 米易县| 丰城市| 繁昌县| 勃利县| 绿春县| 海阳市| 唐山市| 梅州市| 瓦房店市| 南宁市| 抚顺市| 禹城市| 白沙| 鄂尔多斯市| 白银市| 潍坊市| 营山县| 望都县| 江阴市| 汝城县| 砚山县| 乌恰县| 英吉沙县| 曲阳县| 霸州市| 太保市| 乌兰浩特市| 胶州市| 德清县| 永兴县| 罗源县| 梅河口市| 张家川| 恩平市| 敦化市| 名山县|