性猛交XXXX乱大交派对,四虎影视WWW在线观看免费 ,137最大但人文艺术摄影,联系附近成熟妇女

產品展廳收藏該商鋪

您好 登錄 注冊

當前位置:
上海恒遠生物科技有限公司>技術文章>恒遠產品文獻:生物素標記的山羊抗兔抗體引用文獻

技術文章

恒遠產品文獻:生物素標記的山羊抗兔抗體引用文獻

閱讀:720          發(fā)布時間:2021-1-11

【文獻標題】Circular RNA-hsa-circ-0000670 promotes gastric cancer progression through the microRNA-384/SIX4 axis

【作者】Pengliang Liu, Shuang Cai, Nuo Li

【作者單位】中國醫(yī)科大學附屬第四醫(yī)院(the Fourth Affiliated Hospital of China Medical University)

【文獻中引用產品】

生物素標記的山羊抗兔抗體

【關鍵詞】Hsa-circ-0000670,MicroRNA-384,Sine oculis-related homeobox 4,Gastric cancer

【DOI】doi.org/10.1016/j.yexcr.2020.112141

【影響因子(IF)】3.21

出版期刊《Experimental Cell Research》

【產品原文引用】

The tissues were fixed with 4% paraformaldehyde for 24 h, hydrated with 80%, 90%, 100% ethanol and n-butanol, embedded with paraffin, and cut into 5-μm-thick sections. After being heated at 60°C for 1 h, the sections were dewaxed with xylene, dehydrated with gradient ethanol (80%, 90%, 100%) and immersed in 3% H2O2 for 10 min. Antigen retrieval was performed, and the sections were then blocked with 100 μL 5% bovine serum albumin at 37°C for 30 min. Subsequently, the sections were incubated with 100 μL primary rabbit antibodies against SIX4 (1: 2000, ab176713), VEGF (1: 250, ab32152), and CD31 (1: 50, ab28364) overnight at 4°C followed by incubation with a secondary biotin-labeled goat anti-rabbit antibody (HY90046, Shanghai HengYuan Biological Co., Ltd., Shanghai, China) at 37°C for 30 min. Finally, the sections were stained with DAB chromogen (Beijing Bioss Biotechnology Co., Ltd., Beijing, China), and the cell nucleus was stained with hematoxylin for 5 min. The positive cells were stained in brown-yellow. Sections were visualized by Image-Proplus image analysis software (Media Cybernetics, Silver Springs, MD, USA). The target protein expression was evaluated by the proportions of positive cells in total cells detected from 5 randomly selected fields of view on every slide (200-fold magnification). Proportion scores were assigned as follows: positive tumor cells/total tumor cells > 10% positive (+), positive cells < 10% negative (-). 

完整版PDF文獻請咨詢在線客服或者聯(lián)系我司業(yè)務員!

更多公司福利請關注“恒遠生物”VX公眾號!

收藏該商鋪

登錄 后再收藏

提示

您的留言已提交成功!我們將在第一時間回復您~

對比框

產品對比 產品對比 聯(lián)系電話 二維碼 在線交流

掃一掃訪問手機商鋪
13636351217
在線留言
阿合奇县| 富平县| 治多县| 太湖县| 武穴市| 南宁市| 洪雅县| 青神县| 金秀| 井研县| 江孜县| 从江县| 潞西市| 新田县| 荥阳市| 吉安市| 时尚| 耒阳市| 平定县| 河源市| 本溪市| 江津市| 安泽县| 阳新县| 镇宁| 罗源县| 定安县| 鄂尔多斯市| 韶山市| 永胜县| 恭城| 颍上县| 万载县| 潮州市| 安宁市| 察隅县| 盐津县| 连州市| 平邑县| 朝阳县| 教育|