性猛交XXXX乱大交派对,四虎影视WWW在线观看免费 ,137最大但人文艺术摄影,联系附近成熟妇女

產(chǎn)品展廳收藏該商鋪

您好 登錄 注冊

當前位置:
上海恒遠生物科技有限公司>技術(shù)文章>恒遠產(chǎn)品文獻:蝦蛋白酶,淀粉酶,脂肪酶活性ELISA試劑盒引用文獻

技術(shù)文章

恒遠產(chǎn)品文獻:蝦蛋白酶,淀粉酶,脂肪酶活性ELISA試劑盒引用文獻

閱讀:847          發(fā)布時間:2021-1-8

【文獻標題】Dietary prebiotic inulin benefits on growth performance, antioxidant capacity, immune response and intestinal microbiota in Pacific white shrimp (Litopenaeus vannamei) at low salinity

【作者】Li Zhou,Huifeng Li,Jian G. Qin,et.al

【作者單位】海南大學(xué)(Hainan University)

【文獻中引用產(chǎn)品】

蝦蛋白酶(Protease)ELSIA試劑盒

蝦淀粉酶(Amylase)ELISA試劑盒

蝦脂肪酶(Lipase)ELISA試劑盒

【關(guān)鍵詞】Low salinity,Inulin,Growth performance,Antioxidant capacity,Intestinal microbiota,Litopenaeus vannamei

【DOI】doi.org/10.1016/j.aquaculture.2019.734847

【影響因子(IF)】3.42

出版期刊】《Aquaculture》

【產(chǎn)品原文引用】

2.7. Biochemical assays

The entire intestinal of three shrimps mixed as one sample, the hepatopancreas of one shrimp as one sample, each group have six samples. All samples were weighed and homogenized with 9 vol (v/w)of 0.86% physiological saline. Then, the homogenate was centrifuged at 2500 ×g at 4 °C for 10 min and the supernatant was collected for biochemical assays according to the manufacturer's instruction.Activities of intestinal proteaseamylase, and lipase were measured using an enzyme-linked immune sorbent assay (ELISA) kit (Shanghai Hengyuan Biotechnology Co, Ltd) (Li et al., 2019). Phenol oxidase (PO)activity in hepatopancreas was measured using an enzyme-linked immune sorbent assay (ELISA) kit (Nanjing Jiancheng Institute, China).The contents of malondialdehyde (MDA), total superoxide dismutase (T-SOD), catalase (CAT), acid phosphatase (ACP) in hepatopancreas were measured by using specific commercial assay kits (Nanjing Jiancheng Institute, China) (Zhang et al., 2008). Results were recorded on a microplate reader (Epoch, BioTek, USA).Total SOD activity was determined by using WST-1 method. One unit of SOD activity was defined as the amount of enzyme required for 1 mg tissue proteins in 1 mL of a reaction mixture SOD inhibition rates to 50% as monitored at 550 nm.CAT activity was determined by using ammonium molybdate colorimetric method. One unit of CAT activity was defined as 1 mg tissue proteins consumed 1 μmol H2O2 at 405 nm for 60 s. MDA content was determined by using TBA method, which was measured at 532 nm. ACP activity was determined by using colorimetric method. One unit of ACP activity was defined as 1 mg tissue proteins produced 1 mg phenol at 37 °C for 30 min as monitored at 550 nm. Activities of protease, amylase, lipase and phenol oxidase were measured using the enzyme-linked immunosorbent assay (ELISA) and the absorbance (OD) was measured at 450 nm. Total protein content was measured by using Coomassie brilliant blue method.

完整版PDF文獻請咨詢在線客服或者聯(lián)系我司業(yè)務(wù)員!

更多公司福利請關(guān)注“恒遠生物”VX公眾號!

收藏該商鋪

登錄 后再收藏

提示

您的留言已提交成功!我們將在第一時間回復(fù)您~

對比框

產(chǎn)品對比 產(chǎn)品對比 聯(lián)系電話 二維碼 意見反饋 在線交流

掃一掃訪問手機商鋪
13636351217
在線留言
宜兰市| 漠河县| 夏河县| 海盐县| 新蔡县| 哈密市| 阿拉善盟| 阿拉善左旗| 建瓯市| 高唐县| 奉新县| 胶州市| 江源县| 乌兰察布市| 承德市| 德保县| 蚌埠市| 桑日县| 嘉祥县| 环江| 宝清县| 天全县| 霍林郭勒市| 海晏县| 三门县| 荣昌县| 定州市| 瑞安市| 宜昌市| 贵港市| 西昌市| 云南省| 施甸县| 布尔津县| 曲周县| 赞皇县| 敖汉旗| 丹凤县| 连平县| 驻马店市| 丹东市|