性猛交XXXX乱大交派对,四虎影视WWW在线观看免费 ,137最大但人文艺术摄影,联系附近成熟妇女

產(chǎn)品展廳收藏該商鋪

您好 登錄 注冊(cè)

當(dāng)前位置:
上海恒遠(yuǎn)生物科技有限公司>技術(shù)文章>恒遠(yuǎn)產(chǎn)品文獻(xiàn):蝦蛋白酶,淀粉酶,脂肪酶活性ELISA試劑盒引用文獻(xiàn)

技術(shù)文章

恒遠(yuǎn)產(chǎn)品文獻(xiàn):蝦蛋白酶,淀粉酶,脂肪酶活性ELISA試劑盒引用文獻(xiàn)

閱讀:899          發(fā)布時(shí)間:2021-1-8

【文獻(xiàn)標(biāo)題】Dietary prebiotic inulin benefits on growth performance, antioxidant capacity, immune response and intestinal microbiota in Pacific white shrimp (Litopenaeus vannamei) at low salinity

【作者】Li Zhou,Huifeng Li,Jian G. Qin,et.al

【作者單位】海南大學(xué)(Hainan University)

【文獻(xiàn)中引用產(chǎn)品】

蝦蛋白酶(Protease)ELSIA試劑盒

蝦淀粉酶(Amylase)ELISA試劑盒

蝦脂肪酶(Lipase)ELISA試劑盒

【關(guān)鍵詞】Low salinity,Inulin,Growth performance,Antioxidant capacity,Intestinal microbiota,Litopenaeus vannamei

【DOI】doi.org/10.1016/j.aquaculture.2019.734847

【影響因子(IF)】3.42

出版期刊】《Aquaculture》

【產(chǎn)品原文引用】

2.7. Biochemical assays

The entire intestinal of three shrimps mixed as one sample, the hepatopancreas of one shrimp as one sample, each group have six samples. All samples were weighed and homogenized with 9 vol (v/w)of 0.86% physiological saline. Then, the homogenate was centrifuged at 2500 ×g at 4 °C for 10 min and the supernatant was collected for biochemical assays according to the manufacturer's instruction.Activities of intestinal proteaseamylase, and lipase were measured using an enzyme-linked immune sorbent assay (ELISA) kit (Shanghai Hengyuan Biotechnology Co, Ltd) (Li et al., 2019). Phenol oxidase (PO)activity in hepatopancreas was measured using an enzyme-linked immune sorbent assay (ELISA) kit (Nanjing Jiancheng Institute, China).The contents of malondialdehyde (MDA), total superoxide dismutase (T-SOD), catalase (CAT), acid phosphatase (ACP) in hepatopancreas were measured by using specific commercial assay kits (Nanjing Jiancheng Institute, China) (Zhang et al., 2008). Results were recorded on a microplate reader (Epoch, BioTek, USA).Total SOD activity was determined by using WST-1 method. One unit of SOD activity was defined as the amount of enzyme required for 1 mg tissue proteins in 1 mL of a reaction mixture SOD inhibition rates to 50% as monitored at 550 nm.CAT activity was determined by using ammonium molybdate colorimetric method. One unit of CAT activity was defined as 1 mg tissue proteins consumed 1 μmol H2O2 at 405 nm for 60 s. MDA content was determined by using TBA method, which was measured at 532 nm. ACP activity was determined by using colorimetric method. One unit of ACP activity was defined as 1 mg tissue proteins produced 1 mg phenol at 37 °C for 30 min as monitored at 550 nm. Activities of protease, amylase, lipase and phenol oxidase were measured using the enzyme-linked immunosorbent assay (ELISA) and the absorbance (OD) was measured at 450 nm. Total protein content was measured by using Coomassie brilliant blue method.

完整版PDF文獻(xiàn)請(qǐng)咨詢?cè)诰€客服或者聯(lián)系我司業(yè)務(wù)員!

更多公司福利請(qǐng)關(guān)注“恒遠(yuǎn)生物”VX公眾號(hào)!

收藏該商鋪

請(qǐng) 登錄 后再收藏

提示

您的留言已提交成功!我們將在第一時(shí)間回復(fù)您~

對(duì)比框

產(chǎn)品對(duì)比 產(chǎn)品對(duì)比 聯(lián)系電話 二維碼 意見(jiàn)反饋 在線交流

掃一掃訪問(wèn)手機(jī)商鋪
13636351217
在線留言
东辽县| 凯里市| 鄯善县| 屏东市| 澄江县| 长宁区| 万源市| 阿拉善右旗| 天水市| 河池市| 岑巩县| 安康市| 睢宁县| 青川县| 瓮安县| 敦煌市| 密云县| 鹿泉市| 康保县| 桐乡市| 肇源县| 汾西县| 舒兰市| 甘孜| 康乐县| 茶陵县| 虞城县| 金山区| 靖边县| 谷城县| 准格尔旗| 永胜县| 塔河县| 金川县| 五台县| 师宗县| 乌兰浩特市| 马龙县| 克拉玛依市| 太湖县| 通渭县|