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CMCC 51574 費氏志賀氏菌

凍干菌種的復(fù)蘇培養(yǎng)：

1凍干管開封：用浸過7。5%酒精的脫脂棉擦凈凍干管，用火焰加熱其頂端，滴少量無菌水至加熱頂端使之破裂，用銼刀或鑷子敲下已破裂的凍干管頂端

2菌種恢復(fù)培養(yǎng)：用無菌吸管 0.3-0.5毫升適宜到液體培養(yǎng)基，滴入凍干管內(nèi)輕輕振蕩，使凍干菌體溶解呈懸浮狀，吸取全部菌體懸浮液，移植于1-2支建議的斜面培養(yǎng)基上，并在建議的溫度下培養(yǎng)。

CMCC 51574 費氏志賀氏菌 注意事項

1、微生物菌種應(yīng)保藏于低溫、清潔和干燥的地方，室溫放置時間過長會導(dǎo)致菌種衰退；

2、菌種操作應(yīng)在無菌條件下進(jìn)行，防止雜菌污染；

3、斜面菌種保藏時間通常為3-6個月，應(yīng)根據(jù)菌種狀況及時轉(zhuǎn)接；凍干菌種保藏時間通常為2-15年；

Organism:	Escherichia coli (Migula) Caslani and Chalmers
Designations:	EDL 931
Isolation:	feces, human
Depositor:	GK Morris
History:	ATCC <<--GK Morris<<--J.G. Wells
Biosafety Level:	2
Shipped:	freeze-dried
Growth Conditions:	ATCC medium18: Trypticase soy agar Temperature: 37.0°C
Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Antigenic Properties:	serotype O157:H7
Cross References:	Nucleotide (GenBank) : AF305917Escherichia coli O157:H7 nonfunctional beta-glucuronidase (uidA) gene, complete cds.
Comments:	Hemorrhagic colitis [9698] Cytotoxic activity [8277]
References:	8277: Beery JT, et al. Cytotoxic activity of Escherichia coli O157:H7 culture filtrate on the mouse colon and kidney. Curr. Microbiol. 11: 335-342, 1984. 9698: Riley LW, et al. Hemorrhagic colitis associated with a rare Escherichia coli serotype. N. Engl. J. Med. 308: 681-685, 1983. PubMed: 6338386

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