Purmorphamine483367-10-8

Purmorphamine
分子式：C31H32N6O2   分子量：520.62
 
產(chǎn)品描述
Purmorphamine與Smo拮抗劑競(jìng)爭(zhēng)性結(jié)合到Smoothened，并激活Smoothened，IC50為~1.5 μM，也誘導(dǎo)成骨細(xì)胞分化，EC50為1μM。
靶點(diǎn)
Smoothened
         
IC50
~ 1.5 μM
         
體外研究
Purmorphamine activates the Hedgehog pathway by directly binds and activates Smoothened with IC50 of ~ 1.5 μM in compete with cyclopamine, a Smo antagonist.Purmorphamine is a potent inducer of osteogenesis in multipotent C3H10T1/2 cells. The EC50 (based on ALP expression) for Purmorphamine is 1 μM in C3H10T1/2 cells. Purmorphamine (1 μM) and BMP-4 (100 ng/mL) together increase ALP activity more than 90-fold in 3T3-L1 cells.In contrast to BMP-4, Purmorphamine induces osteogenesis by activating Hedgehog signaling in multipotent mesenchymal progenitor cells.
體內(nèi)研究
Purmorphamine up-regulates ALP expression in human mesenchymal stem cell-based constructs on rats.
溶解性
DMSO 4 mg/mL heat，水 <1 mg/mL，乙醇 <1 mg/mL
穩(wěn)定性
2年 -20°C粉狀，6月-80°C溶于DMSO