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Criterion無(wú)染料凝膠成像系統(tǒng)
凝膠分析從2小時(shí)縮短至2.5分鐘，Criterion Stain Free系統(tǒng)拋棄了考馬斯藍(lán)染色和銀染，*顛覆了傳統(tǒng)的蛋白凝膠分析流程，讓我們更輕松地獲得更準(zhǔn)確的結(jié)果。因此，它被評(píng)為2008生命科學(xué)創(chuàng)新產(chǎn)品。
Criterion無(wú)染料凝膠成像系統(tǒng)的主要部件有兩個(gè)：Criterion預(yù)制膠，Criterion無(wú)染料成像儀。
| Comparison of Time Needed for Colloidal Coomassie Staining and Criterion Stain Free Protocols | ||
| Comparison of Time Needed for Colloidal Coomassie Staining and Criterion Stain Free Protocols | ||
| Steps | Colloidal Coomassie Stain | Stain Free |
| | ||
| Washes in deionized water | 15 minutes （3 x 5 minute） | 0 minutes |
| Staining time | 60 minutes （30–60 minutes） | 0 minutes |
| Destaining time | 30 minutes | 0 minutes |
| Destaining time | 30 minutes | 0 minutes |
| Imaging and analysis | 15–30 minutes | 2.5–5 minutes |
| | ||
| Total time | 135 minutes | <5 minutes |
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Criterion Stain Free system vs. Coomassie staining: Duplicate serial dilutions of molecular weight standards were visualized using the Criterion Stain Free system and Coomassie staining. The Criterion Stain Free system SDS-PAGE provides reproducible results in less than 5 minutes. Coomassie staining produces variable background despite use of consistent staining and destaining times.
Criterion Stain Free Gels
Criterion Stain Free gels have Tris-HCl formulation for PAGE applications. Criterion Stain Free gels are made without SDS. With SDS omitted from the running buffer, the gels can be used to run proteins under nondenaturing conditions for subsequent analysis of native conformation and activity. To run denaturing gels, simply use a running buffer that contains SDS.
Criterion Stain Free gels have Tris-HCl formulation for PAGE applications. Criterion Stain Free gels are made without SDS. With SDS omitted from the running buffer, the gels can be used to run proteins under nondenaturing conditions for subsequent analysis of native conformation and activity. To run denaturing gels, simply use a running buffer that contains SDS.
| Configurations of Criterion Stain Free Gels | ||
| | ||
| Gradient | Wells | Sample Volume |
| | ||
| 10% resolving gel | 12 + 2 | 45 µl |
| 10% resolving gel | 18 | 30µl |
| 4–20 % linear gradient | 12+2 | 45 µl |
| 4–20 % linear gradient | 18 | 30 µl |
| 4–20 % linear gradient | 26 | 15 µl |
| 8–16% linear gradient | IPG + 1 well | 11 cm IPG Strip |
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Single-percentage resolving gels — Choose a single-percentage gel when your sample has a limited size range of molecules and your goal is to separate a single band from neighboring bands. These gels will produce the greatest separation between bands with similar molecular weights. Single-percentage gels are cast with a 4% stacking gel to further sharpen protein bands before they enter the resolving gel.
Linear gradient gels — Choose a linear gradient gel if your sample contains a wide range of molecular weights. These gels allow both high and low molecular weight bands to be visualized on the same gel.
Criterion Stain Free Imager
The Criterion Stain Free imager is a compact instrument with a sample tray for loading the precast gels and a start button to initiate automated protein visualization and analysis.
Designed with feedback from scientists involved in biopharmaceutical research, the imager is small, practical, and aesthetically pleasing. It is designed with user-friendly features such as the power button and serial number located on the front of the instrument, a four-step quick guide displayed on the sample tray, and a slip-free tray handle. The Criterion Stain Free imager is a simple and easy-to-use platform designed with the customer in mind from start to finish.
Image Lab Software
Image Lab software is designed for one-touch SDS-PAGE imaging and analysis automation in the Criterion Stain Free system. Life science researchers helped create Image Lab software, resulting in an easy-to-use interface that offers multiple image optimization and analysis features including molecular weight determination and purity assessment. Researchers can save imaging and analysis settings in Protocols, and with the touch of the start button on the Criterion Stain Free imager, Image Lab software choreographs the automated protein signal activation, gel imaging, and analysis to quickly produce high-quality, reproducible results.