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CYT-234

重組人干擾素-β 1b （rHuIFN-β1b）

2μg/10μg/1mg

Prospec

Background:
	One of the type I interferons produced by fibroblasts in response to stimulation by live or inactivated virus or by double-stranded RNA. It is a cytokine with antiviral, antiproliferative, and immunomodulating activity. The synthesis of IFN-beta can be induced by common inducers of interferons, including viruses, double-stranded RNA, and micro-organisms. It is induced also by some cytokines such as TNF and IL-1 .
	Description :
	Recombinant Human IFN-beta 1b produced in E.Coli is a single, non-glycosylated mutein （variant form） of human Interferon beta-1b polypeptide chain containing 165 amino acids and having a molecular mass of 18510.86 Dalton. The Interferon beta gene was cloned from human fibroblasts and altered to substitute Serine for the Cysteine residue found at position 17. IFN-beta-1b is purified by proprietary chromatographic techniques.
	Physical Appearance:
	Sterile Filtered White lyophilized （freeze-dried） powder.
	Formulation:
	1mg/ml solution containing 50mg Human Albumin & 50mg dextrose.
	Solubility:
	It is recommended to reconstitute the lyophilized Interferon beta-1b in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
	Stability:
	Lyophilized Interferon beta-1b although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution,liquid form of IFN-beta-1b should be stored at 4 C up to 3 weeks and for future use below -18 C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please avoid freeze-thaw cycles.
	Purity:
	Greater than 98.0% as determined by: （a） Analysis by RP-HPLC. （b） Anion-exchange FPLC. （c） Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
	Amino acid sequence:
	The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Tyr-Asn-Leu-Leu.
	Dimers and aggregates:
	Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
	Biological Activity:
	ProSpec's Interferon beta-1b is fully biologically active when compared to standard. The specific activity as determined in a viral resistance assay （human "Wish" cell line and VSV virus or the monkey VERO cell line with EMCV virus） was found to be 32 x 10 IU/mg. ⁶ IU/ mg.
	Endotoxin:
	Less than 0.1 ng/µg （IEU/µg） of IFN-beta-1b.
	Protein content:
	Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 1.493 as the extinction coefficient for a 0.1% （1mg/ml） solution. This value is calculated by the PC GENE computer analysis program of protein sequences （InliGenetics）. 2. Analysis by RP-HPLC, using a standard solution of IFN-beta as a Reference Standard.
	Usage:
	Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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