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人胚腎細胞;293 Cells, low passage
上皮細胞
貼壁生長
293細胞是人胚胎腎細胞用剪切過的腺病毒5 DNA轉化得到。它們包含并表達腺病毒5的早期區(qū)段1。 它們包含并表達腺病毒5的早期區(qū)段1。 人胚腎細胞;293 Cells, low passage它們能互補E1失活的腺病毒突變株和載體,使其生長。 在DNA轉染檢測中它們是特別有效的受體細胞。 對于大多數即使不是全部人腺病毒的生長和溶菌斑測試,它們也表現很好。 這些細胞廣泛應用于因缺失或代換E1序列引起的E1失活突變株和腺病毒載體的繁殖與滴定。 因此它們被用來構建腺病毒載體。 用于構建腺病毒載體使用低代數細胞,一般不超過40代。 對于保持這株細胞的優(yōu)良特性,正確
MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle's Balanced Salts) 胎牛血清,10%
消化3-5分鐘。1:2。3天內可長滿。
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*培養(yǎng)基+8%DMSO
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