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SpeciesReactivity
Mouse

Samples
Serum. Plasma, Culture medium and other fluids may be adapted

KitContents
1. Purified Sm/nRNP Coated Microwells (96 wells) 5411 2. Mouse Anti-nRNP negative serum, control 1 ml 5412 3. Mouse Anti-nRNP positive serum, control 1 ml 5413 4. Goat Anti-mouse IgG (H+L)-HRP Conjugate (100X), 0.2 ml 5414 5. Sample Diluent (10X), 10ml SD-10 6. Wash Solution. (100X), 10 ml WB-100 7. TMB Substrate Solution, 12 ml 80091 8. Stop Solution, 12 ml 80101 9. Complete Instruction Manual M-5410

SpCrossreactivity
The nRNP antigen, used for coating ELISA plates, has been purified from calf and/or rabbit thymus cytosol through a combination of immunoaffinity chromatography (anti-nRNP-Sepharose) and other biochemical techniques. The purified preparation does not contain any detectable SS-A, SS-B, Scl-70, and Jo-1 antigens by sensitive ELISA technique. This kit detects anti-nRNP Ig's (IgG+A+M) in mouse serum. It is possible to use isotype specific anti-mouse Ig, such as anti-mouse IgM or anti-mouse IgG1-HRP, to measure isotype specific antibodies.

MSDS
TMB (substrate), H2SO4 (1% in a buffered stop solution), and Prolcin-300 (0.1% v/v in standards, sample diluent and HRP-conjugates

For research use only, not for diagnostic or therapeutic use.