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KitContents
Please refer to complete instructions manual supplied with the product

SpeciesReactivity
all species

Form
Stds=0-25 ug/dL; Sample=25 ul; Sensitivity= 0.5 ug/dL; 90 min assay; Quantitative, Competitive assay

Samples
Serum. T4 assay in Plasma (EDTA) have also been used (see published references). Other fluids may be adapted

SpCrossreactivity
The specificity of total T4 ELISA kit was determined by measuring interference from high concentrations of 3,5-diiodothyronine (up to 10 ug/dL), 3,3',5-triiodothyronine (rT3,,up to 1000 ng/ml), 3,3',5-triiodothyronine (T3,,up to 59 nmol/l), 3,3',5-triiodothyroacetic acid (up to 42 nmol/l), 3,3',5-triiodothyropropionic acid (up to 63 nmol/l), Aspirin (10 mg/dL) iodoacetic acid (10 ug/dL), phenylbutazone (10 mg/dL). No significant cross-reaction was observed with any of these compounds. T4 is the same in human and animals. ADI human T4 elisa Kit has been used in mouse and rat samples (see publications).

MSDS
TMB (substrate), H2SO4 (1% in a buffered stop solution), and Prolcin-300 (0.1% v/v in standards, sample diluent and HRP-conjugates

For research use only, not for diagnostic or therapeutic use