ExoView外泌體全面表征試劑盒

外泌體計(jì)數(shù)、粒徑、蛋白表達(dá)、蛋白共定位次完成

實(shí)驗(yàn)原理

① 35 μL外泌體樣品滴加在芯片上孵育；

② 預(yù)包被的抗體異結(jié)合外泌體表面蛋白以捕獲外泌體；

③ 再使用熒光抗體異性標(biāo)記需要表征的標(biāo)記物；

④ 后用ExoView R100檢測(cè)外泌體粒徑、計(jì)數(shù)、蛋白表達(dá)（CD9，CD81，CD63等）及共定位。

檢測(cè)流程

產(chǎn)品類別

試劑盒點(diǎn)

測(cè)試數(shù)據(jù)

外泌體熒光數(shù)量統(tǒng)計(jì)

外泌體粒徑檢測(cè)

外泌體內(nèi)容物檢測(cè)

熒光強(qiáng)度與粒徑關(guān)系

熒光共定位分析

發(fā)表文章

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• Kyoung-Won Ko.(2021) Integrated Bioactive Scaffold with Polydeoxyribonucleotide and Stem-Cell-Derived Extracellular Vesicles for Kidney Regeneration. ACS Nano. 

• Tanina Arab. (2021) Characterization of extracellular vesicles and synthetic nanoparticles with four orthogonal single‐particle analysis platforms. Journal of Extracellular Vesicles. 

• Niaz Z.Khan.(2021) Spinal cord injury alters microRNA and CD81+ exosome levels in plasma extracellular nanoparticles with neuroinflammatory potential. Brain, Behavior, and Immunity. 

• Dario Brambilla. (2021) EV Separation: Release of Intact Extracellular Vesicles Immunocaptured on Magnetic Particles. Analytical Chemistry. 

• Enkhtuya Radna. (2021) Extracellular vesicle mediated feto-maternal HMGB1 signaling induces preterm birth. Lab on a Chip. 

• Li, M., Soder. (2021) WJMSC‐derived small extracellular vesicle enhance T cell suppression through PD‐L1. Journal of Extracellular Vesicles. 

• Crescitelli, R. (2021) Isolation and characterization of extracellular vesicle subpopulations from tissues. Nature protocols. 

• Berger, A. (2021). Local administration of stem cell-derived extracellular vesicles in a thermoresponsive hydrogel promotes a pro-healing effect in a rat model of colo-cutaneous post-surgical fistula. Nanoscale. 

• Vidal, M. (2020) Exosomes and GPI-anchored proteins: Judicious pairs for investigating biomarkers from body fluids. Advanced drug delivery reviews. 

• K Cho, H Kook.(2020)Study of immune-tolerized cell lines and extracellular vesicles inductive environment promoting continuous expression and secretion of HLA-G from semiallograft immune tolerance during pregnancy. Journal of Extracellular Vesicles. 

• Maximillian A. Rogers.(2020)Annexin A1–dependent tethering promotes extracellular vesicle aggregation revealed with single–extracellular vesicle analysis. Cell Biology. 

• Annette M. Marleau.(2020)Targeting tumor-derived exosomes using a lectin affinity hemofiltration device. Cancer Research. 

• Alessandro Gori.(2020)Membrane-Binding Peptides for Extracellular Vesicles On-Chip Analysis. Journal of Extracellular Vesicles. 

• Rossella Crescitelli.(2020)Subpopulations of extracellular vesicles from human metastatic melanoma tissue identified by quantitative proteomics after optimized isolation. Journal of Extracellular Vesicles. 

• Maria S. Panagopoulou.(2020) Phenotypic analysis of extracellular vesicles:  a review on the applications of fluorescence. Journal of Extracellular Vesicles.

• WeiYan.(2020) Immune Cell-Derived Exosomes in the Cancer-Immunity Cycle. Trends in Cancer. 

• Daniel Bachurski. (2019) Small RNA Sequencing across Diverse Biofluids Identifies Optimal Methods for exRNA Isolation. Cell.

用戶單位