微流控芯片上的器官（僅芯片）-魯爾

描述

一個(gè)理想的芯片上的器官應(yīng)該易于使用，并且可以可逆地進(jìn)入芯片中的細(xì)胞進(jìn)行下游分析。因此，我們的芯片器官滿足所有這些要求模塊化微流控芯片由兩塊PC（聚碳酸酯）塑料板組成，每個(gè)板上有四塊可逆組裝用集成磁鐵和一個(gè)彈性PDMS薄膜，以確保良好的密封性。芯片上部的luer連接器允許藥物篩選和/或細(xì)胞分化實(shí)驗(yàn)期間的介質(zhì)灌注和動(dòng)態(tài)流動(dòng)條件。

不同的貼片可用于培養(yǎng)細(xì)胞或在培養(yǎng)皿或微孔板中分化人誘導(dǎo)多能干細(xì)胞（hiPSC），以創(chuàng)建組織貼片（如心臟、神經(jīng)元等）然后，獲得的組織貼片（或其他組織培養(yǎng)插入物）可以集成到芯片上組裝的器官中。文化修補(bǔ)程序在這里可用！

微腔和微通道在芯片的兩個(gè)塑料板上形成圖案，形成兩個(gè)獨(dú)立的微流控系統(tǒng)：由組織貼片分離的上腔和下腔。這兩個(gè)微流控室可以用不同的培養(yǎng)基進(jìn)行灌注。從而形成組織界面，模擬肺泡、胃、腸、腎、肝、腦血、皮膚功能等。

芯片內(nèi)的組織可以通過(guò)顯微鏡觀察或采集用于下游分析。

微流控芯片

該芯片設(shè)計(jì)用于各種類型貼片的可逆集成。它由兩塊塑料板組成，每一塊都有一個(gè)彈性薄膜、四個(gè)嵌入磁鐵、預(yù)圖案化的腔室和微通道當(dāng)貼片放置在兩塊板之間時(shí)，四對(duì)磁鐵之間的力確保在高達(dá)8千帕的壓力下自動(dòng)裝配后，標(biāo)準(zhǔn)的luer連接板上允許一個(gè)簡(jiǎn)單的介質(zhì)和藥物灌注。

為確保高流量壓力（高達(dá)100千帕）下的良好密封性，芯片可放置在手動(dòng)螺紋夾具中進(jìn)行組裝和快速操作（不包括夾具，可單獨(dú)訂購(gòu)；單擊此處了解更多信息）。在動(dòng)態(tài)流動(dòng)條件下的實(shí)驗(yàn)結(jié)束時(shí)，通過(guò)拆開(kāi)夾子和微流控芯片，可以很容易地取出組織補(bǔ)片。

更普遍地說(shuō)，將4個(gè)微流控芯片與不同的組織貼片集成，可以創(chuàng)建一個(gè)類似于身體的循環(huán)或微生理系統(tǒng)，以研究不同組織（芯片上的身體）的相互影響。例如，可以將人的腸、肝、皮膚和腎細(xì)胞與一個(gè)中等循環(huán)回路聯(lián)合培養(yǎng)，這將為藥物篩選提供一個(gè)可靠的平臺(tái)。

微流控芯片上的器官（僅芯片）-魯爾

Description

An ideal Organ-on-Chip should be easy to use and with a reversible access to cells in chip for downstream analysis. Hence our Organ-on-Chip meets all these demands. The modular microfluidic chip consists in two plastic plates in PC (Polycarbonate), each having four integrated magnets for reversible assembly and an elastomeric PDMS thin film to ensure good sealing. The Luer Connectors in the upper part of the chip allow medium perfusion and dynamic flow conditions during your experiments of drug screening and/or cell differentiation.

Different patches can be used to culture cells or to differentiate Human Induced Pluripotent Stem Cells (hiPSC) in dish or microplates in order to create your tissue patch (e.g. cardiac, neuron, etc). Then, the obtained tissue patch (or other tissue culture inserts) can be integrated in the assembled Organ-on-Chip. Culture patches are available here!

Micro-chambers and micro-channels are patterned in both plastic plates of the chip to create two independent microfluidic systems: the upper and the lower chambers separated by the tissue patch. These two microfluidic chambers can be perfused with different culture media. Thus a tissue interface can be formed to simulate alveolar, stomach, intestine, kidney, liver, brain-blood, skin functions, etc.

Tissues inside the chip can be observed by microscopy or harvested for downstream analysis. 

Microfluidic chip

This chip was designed for reversible integration of various types of patches. It consists in two plastic plates, each having an elastomeric thin film, four embedded magnets, and pre-patterned chambers and micro-channels. When a patch is placed between the two plates, the force between the four pairs of magnets ensures auto-assembling at pressures up to 8 kPa. Finally, the standard Luer Connectors of the upper plate allow an easy medium and drug perfusion.

To ensure good sealing with high flow pressures (up to 100 kPa), the chip can be placed in a hand screwed clamp for assembling and fast operation (the clamp is not included and can be ordered separately; click here for additional information). At the end of your experiment in dynamic flow conditions, the tissue patch can be easily removed by dissembling the clamps and the microfluidic chip. 

More generally, integration of 4 microfluidic chips with different tissue patches will allow creating a body-like circulatory or micro-physiological system to investigate the mutual influences of different tissues (a Body-on-Chip). For example, a co-culture of human intestine, liver, skin and kidney cells with a medium circulation loop could be assembled and it will represent a reliable platform for drug screening.