3D Cell Culture Chip三維細(xì)胞培養(yǎng)芯片
- 公司名稱 世聯(lián)博研（北京）科技有限公司
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3D Cell Culture Chip三維細(xì)胞培養(yǎng)芯片交互作用DDI芯片細(xì)胞培養(yǎng)芯片微流控芯片
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| 價(jià)格區(qū)間 | 面議 | 儀器種類 | 微流控芯片系統(tǒng) |
|---|---|---|---|
| 應(yīng)用領(lǐng)域 | 生物產(chǎn)業(yè) |
3D Cell Culture Chip三維細(xì)胞培養(yǎng)芯片
ddi芯片是一種微流控器件，通過它可以在不同的距離上研究細(xì)胞和因子之間的相互作用。這提供了一個(gè)更生理的設(shè)置，并允許同時(shí)調(diào)查不同的距離在同一設(shè)備。使用DDI芯片還可以測(cè)試影響距離相關(guān)交互的代理。
材料
ddi芯片由生物相容性聚二甲基硅氧烷（pdms）制成，具有透氣性，保證了co2和o2的交換。薄玻璃載體是一種具有高透明、低自熒光等優(yōu)良光學(xué)性能的顯微鏡載玻片。這允許通過光學(xué)顯微鏡、熒光顯微鏡和共焦顯微鏡以時(shí)間和空間分辨率直接觀察生物樣品。
技術(shù)特點(diǎn)
模式包括：
2個(gè)營(yíng)養(yǎng)液供應(yīng)和受試化合物注射的介質(zhì)通道（外部通道）；
2個(gè)基質(zhì)通道（中間通道），用于細(xì)胞接種或不接種水凝膠；
1主中央通道，寬度沿其長(zhǎng)度逐漸增加
邊介質(zhì)通道中間矩陣通道中間通道
高度150微米150微米150微米
寬度2500微米
1125微米（狹窄部分）
3375微米（寬部分）
從1250微米逐漸變?yōu)?000微米
體積（建議注射）
20微升20微升20微升
入口直徑1000微米1000微米1000微米1000微米
DDI芯片沒有涂層表面。根據(jù)您的應(yīng)用，并終改善細(xì)胞粘附性，您可以使用聚合物和/或蛋白質(zhì)溶液來覆蓋通道表面。
這種微流控裝置還可以與細(xì)胞嵌入水凝膠（如I型膠原、IV型膠原、纖維蛋白、基質(zhì)凝膠等）中，以重建可靠的生物微環(huán)境。
入口直徑為1毫米，可通過普通吸管輕松注入細(xì)胞懸液20微升是每個(gè)通道/腔室的推薦體積；這將允許在實(shí)際注射和*填充時(shí)有少量額外的微升。
此外，還可以使用直接安裝在入口的1/16“管子在動(dòng)態(tài)流動(dòng)條件下進(jìn)行研究。
收益
使用方便
生理相關(guān)微環(huán)境
單細(xì)胞分辨率
直接可視化
時(shí)空分辨率
定性和定量評(píng)估
3D Cell Culture Chip三維細(xì)胞培養(yǎng)芯片
The DDI-Chip is a microfluidic device through which the interaction between cells and factors can be investigated at different distances from each other. This provides a more physiological setup and allows simultaneous investigation of different distances in the same device. With the DDI-Chip it is also possible to test agents that can affect the distance dependent interactions.
Materials
The DDI-Chip is made of biocompatible polydimethylsiloxane (PDMS) and is gas-permeable to ensure the CO2 and O2 exchange. The support in thin glassis a microscope slide with excellent optical properties such as high transparency and low autofluorescence. This allows a direct observation of your biological sample with temporal and spatial resolution by using optical, fluorescent and confocal microscopes.
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Technical features
The pattern consists in:
- 2 medium channels (the external ones) for nutrient supply and tested compounds injection;
- 2 matrix channels (the intermediate ones) for cell seeding with or w/o hydrogels;
- 1 main central channel with an width that increases progressively along its length
| Side Medium Channels | Intermediate Matrix Channels | Middle Channel | |
| Height | 150 µm | 150 µm | 150 µm |
| Width | 2500 µm | 1125 µm (the narrow parts) 3375 µm (the wide parts) | Gradually changed from 1250 µm to 5000 µm |
Volume (recommended for injection) | 20 µL | 20 µL | 20 µL |
| Diameter of inlets | 1000 µm | 1000 µm | 1000 µm |
The DDI-Chip has not coated surfaces. According to your application and to eventually improve cell adhesion, you can coat the channel surface by using your solutions of polymers and/or proteins.
This microfluidic device can be used also with cells embedded in hydrogels such as Collagen I, Collagen IV, Fibrin, Matrigel, etc. to recreate a reliable biological microenvironment.
The inlet diameter of 1 mm allows an easy injection of the cell suspension through common pipettes. 20 µL is the recommended volume for each channel/chamber; this will allow to have few extra µL for a practical injection and complete filling.
Additionally, it would be possible to perform studies in dynamic flow conditions by using 1/16” tubing directly fitted in the inlets.
Benefits
- Easy-to-use
- Physiologically relevant microenvironment
- Single cell resolution
- Direct visualization
- Temporal and spatial resolution
- Qualitative and quantitative assessments
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