3D Cell Culture Chip 三維細(xì)胞培養(yǎng)芯片
- 公司名稱 世聯(lián)博研（北京）科技有限公司
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- 更新時間 2019/11/4 16:11:13
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3D Cell Culture Chip三維細(xì)胞培養(yǎng)芯片細(xì)胞培養(yǎng)芯片微流控芯片侵襲趨化IC芯片
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| 價格區(qū)間 | 面議 | 儀器種類 | 微流控芯片系統(tǒng) |
|---|---|---|---|
| 應(yīng)用領(lǐng)域 | 生物產(chǎn)業(yè) |
3D Cell Culture Chip 三維細(xì)胞培養(yǎng)芯片
材料
芯片采用生物相容性聚二甲基硅氧烷（PDMS）制成，具有透氣性，保證了CO2和O2的交換薄玻璃載體是一種具有高透明、低自熒光等優(yōu)良光學(xué)性能的顯微鏡載玻片。這允許通過光學(xué)顯微鏡、熒光顯微鏡和共焦顯微鏡以時間和空間分辨率直接觀察生物樣品。
技術(shù)特點(diǎn)
邊溝中間溝
高度150微米150微米
寬度
3250微米
1125微米（狹窄部分）
3375微米（寬部分）
體積（建議注射）
20微升20微升
入口直徑1000微米1000微米
芯片表面沒有涂層。根據(jù)您的應(yīng)用，并終改善細(xì)胞粘附性，您可以使用聚合物和/或蛋白質(zhì)溶液來覆蓋通道表面。
這種微流控裝置還可以與細(xì)胞嵌入水凝膠（如I型膠原、IV型膠原、纖維蛋白、基質(zhì)凝膠等）中，以重建可靠的生物微環(huán)境。
入口直徑為1毫米，可通過普通吸管輕松注入細(xì)胞懸液20微升是每個通道/腔室的推薦體積；這將允許在實(shí)際注射和*填充時有少量額外的微升。
此外，還可以使用直接安裝在入口的1/16“管子在動態(tài)流動條件下進(jìn)行研究。
好處
使用方便
生理相關(guān)微環(huán)境
單細(xì)胞分辨率
直接可視化
時空分辨率
定性和定量評估
3D Cell Culture Chip 三維細(xì)胞培養(yǎng)芯片
Materials
The IC-Chip is made of biocompatible polydimethylsiloxane (PDMS) and is gas-permeable to ensure the CO2 and O2 exchange. The support in thin glass is a microscope slide with excellent optical properties such as high transparency and low autofluorescence. This allows a direct observation of your biological sample with temporal and spatial resolution by using optical, fluorescent and confocal microscopes.
 |  |
Technical features
| Side Channels | Middle Channel | |
| Height | 150 µm | 150 µm |
| Width | 3250 µm | 1125 µm (the narrow parts) 3375 µm (the wide parts) |
Volume (recommended for injection) | 20 µL | 20 µL |
| Diameter of inlets | 1000 µm | 1000 µm |
The IC-Chip has not coated surfaces. According to your application and to eventually improve cell adhesion, you can coat the channel surface by using your solutions of polymers and/or proteins.
This microfluidic device can be used also with cells embedded in hydrogels such as Collagen I, Collagen IV, Fibrin, Matrigel, etc. to recreate a reliable biological microenvironment.
The inlet diameter of 1 mm allows an easy injection of the cell suspension through common pipettes. 20 µL is the recommended volume for each channel/chamber; this will allow to have few extra µL for a practical injection and complete filling.
Additionally, it would be possible to perform studies in dynamic flow conditions by using 1/16” tubing directly fitted in the inlets.
Benefits
- Easy-to-use
- Physiologically relevant microenvironment
- Single cell resolution
- Direct visualization
- Temporal and spatial resolution
- Qualitative and quantitative assessments
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