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化工儀器網(wǎng)>產(chǎn)品展廳>配件耗材>生化/分子生物配件/附件>培養(yǎng)板/微孔板/滴定板> flexcell SpillGuard®培養(yǎng)板, Spill...

flexcell SpillGuard®培養(yǎng)板, SpillGuard® cul

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世聯(lián)博研(北京)科技有限公司(Bio Excellence International Tech Co.,Ltd)簡(jiǎn)稱為世聯(lián)博研。世聯(lián)博研是一家集進(jìn)口科研儀器代理銷售以及實(shí)驗(yàn)技術(shù)服務(wù)于一體的技術(shù)公司。世聯(lián)博研專注生物力學(xué)和3D生物打印前沿科研設(shè)備代理銷售及科研實(shí)驗(yàn)項(xiàng)目合作服務(wù),內(nèi)容涵蓋了血管力學(xué)生物學(xué)、生物力學(xué)建模仿真與應(yīng)用、細(xì)胞分子生物力學(xué)、組織修復(fù)生物力學(xué)、骨與關(guān)節(jié)生物力學(xué)、口腔力學(xué)生物學(xué)、眼耳鼻咽喉生物力學(xué)、康復(fù)工程生物力學(xué)、生物材料力學(xué)與仿生學(xué)、人體運(yùn)動(dòng)生物力學(xué)等生物力學(xué)研究以及生物材料打印、打印樣品生物力學(xué)性能測(cè)試分析的前沿領(lǐng)域科研利器和科研服務(wù)。

世聯(lián)博研的客戶范圍:
科研院所單位、生物醫(yī)學(xué)科研高校、醫(yī)院基礎(chǔ)科研單位等。

世聯(lián)博研公司代理的品牌具有:
1)近10年長(zhǎng)期穩(wěn)定的貨源
2)以生物力學(xué)、細(xì)胞力學(xué)、細(xì)胞生物分子學(xué)、生物醫(yī)學(xué)組織工程、生物材料學(xué)為主,兼顧其他相關(guān)產(chǎn)品線
3)提供專業(yè)產(chǎn)品培訓(xùn)和銷售培訓(xùn)
4)良好的技術(shù)支持
5)已成交老客戶考證
6)每年新增的貨源。

細(xì)胞應(yīng)力加載儀,3細(xì)胞打印機(jī),NanoTweezer新型激光光鑷系統(tǒng),PicoTwist磁鑷,美國(guó)NeuroIndx品牌Kuiqpick單細(xì)胞捕獲切割系統(tǒng)

供貨周期 現(xiàn)貨

 

BioFlex雙向拉應(yīng)力培養(yǎng)板, Uniflex單向拉應(yīng)力培養(yǎng)板 、TissueTrain三維細(xì)胞組織培養(yǎng)板等系列細(xì)胞培養(yǎng)一起使用,
培養(yǎng)板類型、包被表面材料豐富:Amino, Collagen (Type I or IV), Elastin, ProNectin (R GD), Laminin (YIGSR).表面涂層豐富的
包被材料, 您可以跟根據(jù)不同細(xì)胞組織可以靈活選擇不同包被材料表面 (包被材料選擇參考)

該應(yīng)力加載系統(tǒng)配套培養(yǎng)板zui大伸展率
Culture Plate for tension systemMax Achievable Strain
BioFLEX®雙向應(yīng)力細(xì)胞培養(yǎng)板(BioFLEX®CULTURE PLATES)21.80%
Tissue Train三維細(xì)胞組織培養(yǎng)板(Tissue Train culture plates)20.80%
UniFlex™單向應(yīng)力細(xì)胞培養(yǎng)板(UniFlex™Culture Plates)12.20%
24孔高通量BIOFLEX®培養(yǎng)板培養(yǎng)板(HT BIOFLEX®CULTURE PLATES)15.00%
No Loading Stations33%

1、BioFLEX®雙向應(yīng)力細(xì)胞培養(yǎng)板(BioFLEX®CULTURE PLATES)
雙向應(yīng)力細(xì)胞培養(yǎng)板,BioFLEX CULTURE PLATES

BIOFLEX®CULTURE PLATES(BIOFLEX®培養(yǎng)板培養(yǎng)板)

編號(hào)產(chǎn)品

產(chǎn)品名稱

BF-3001U-Case(每箱40塊)BF-3001E-Each(每塊)BioFlex Culture Plate-Untreated(6孔表面未處理的BioFLEX®雙向應(yīng)力細(xì)胞培養(yǎng)板)
BF-3001A-Case(每箱40塊)BF-3001A-Each(每塊)BioFlex Culture Plate-Amino (6孔氨基BioFLEX®雙向應(yīng)力細(xì)胞培養(yǎng)板)
BF-3001C-Case(每箱40塊)BF-3001C-Each(每塊)BioFlex Culture Plate-Collagen Type I (6孔膠原I 型BioFLEX®雙向應(yīng)力細(xì)胞培養(yǎng)板)
BF-3001C(IV)-Case(每箱40塊)BF-3001C(IV)-Each(每塊)BioFlex Culture Plate-Collagen Type IV (6孔膠原IV型BioFLEX®雙向應(yīng)力細(xì)胞培養(yǎng)板)
BF-3001E-Case(每箱40塊)BF-3001E-Each(每塊)BioFlex Culture Plate-Elastin (6孔彈力BioFLEX®雙向應(yīng)力細(xì)胞培養(yǎng)板)
BF-3001P-Case(每箱40塊)BF-3001P-Each(每塊)BioFlex Culture Plate-ProNectin (6孔 ProNectin BioFLEX®雙向應(yīng)力細(xì)胞培養(yǎng)板)
BF-3001L-Case(每箱40塊)BF-3001L-Each(每塊)BioFlex Culture Plate-Laminin (6孔層粘連蛋白BioFLEX®雙向應(yīng)力細(xì)胞培養(yǎng)板)

2、UniFlex™單向應(yīng)力細(xì)胞培養(yǎng)板(UniFlex™Culture Plates)

flexcell,UniFlex(flexcell雙向應(yīng)力培養(yǎng)板)

  • BioFlex細(xì)胞培養(yǎng)板和FX-5000T應(yīng)力加載系統(tǒng)配套使用,為細(xì)胞提供應(yīng)力加載。
  • 孔板底部是由彈性硅膠模制作而成,柔韌性好,透明度高。
  • 6孔圓形,總生長(zhǎng)面積57.75 cm2(9.62 cm2/孔).
  • 孔板底部的硅膠模超薄,厚度僅為0.020",(0.0508cm)可以使用標(biāo)準(zhǔn)正立式顯微鏡/倒立顯微鏡觀察細(xì)胞。
  • 五種不同包被的培養(yǎng)表面:Amino, Collagen (Type I or IV), Elastin, ProNectin (R GD), Laminin (YIGSR).
    Covalently bound matrix surfaces: Amino, Collagen (Type I or IV), Elastin, Pronectin®(RGD), and Laminin (YIGSR).(包被材料選擇參考)
  • 光學(xué)性能佳,自身熒光低。
  • 當(dāng)與圓柱形加載平臺(tái)共同使用時(shí),可以對(duì)細(xì)胞進(jìn)行均勻的徑向和圓周應(yīng)力加載。
  • UniFlex細(xì)胞培養(yǎng)板和FX-5000T應(yīng)力加載系統(tǒng)和TissueTrain細(xì)胞培養(yǎng)系統(tǒng)配套使用。
  • 準(zhǔn)確測(cè)量應(yīng)力---負(fù)壓系統(tǒng)曲線,保證應(yīng)力的準(zhǔn)確性和可重復(fù)性。
  • 單軸向應(yīng)力作用于寬0.6英寸x0.952英寸(3.68 cm2)的長(zhǎng)方形區(qū)域中。
  • 單軸向應(yīng)力變幅在+/-1.5%.
  • 五種不同包被的培養(yǎng)表面:Amino, Collagen (Type I or IV) Elastin, ProNectin (RGD), Laminin (YIGSR)
    (包被材料選擇參考)
  • 為獲得準(zhǔn)確的單軸向應(yīng)力,必須和弧矩形應(yīng)力加載平臺(tái)配套使用。
    編號(hào)產(chǎn)品產(chǎn)品名稱
    UF-4001U-Case(每箱40塊)UF-4001U-Each(每塊)UniFlex Culture Plate-Untreated(6孔表面未處理的BioFLEX®雙向應(yīng)力細(xì)胞培養(yǎng)板)
    UF-4001A-Case(每箱40塊)UF-4001A-Each(每塊)UniFlex Culture Plate-Amino(6孔氨基UniFlex™單向應(yīng)力細(xì)胞培養(yǎng)板)
    UF-4001C-Case(每箱40塊)UF-4001C-Each(每塊)UniFlex Culture Plate-Collagen Type I(6孔膠原I 型UniFlex™單向應(yīng)力細(xì)胞培養(yǎng)板)
    UF-4001C(IV)-Case(每箱40塊)UF-4001C(IV)-Each(每塊)UniFlex Culture Plate-Collagen Type IV(6孔膠原IV型UniFlex™單向應(yīng)力細(xì)胞培養(yǎng)板)
    UF-4001E-Case(每箱40塊)UF-4001E-Each(每塊)UniFlex Culture Plate-Elastin (6孔彈力UniFlex™單向應(yīng)力細(xì)胞培養(yǎng)板)
    UF-4001P-Case(每箱40塊)UF-4001P-Each(每塊)UniFlex Culture Plate-ProNectin (6孔ProNectin UniFlex™單向應(yīng)力細(xì)胞培養(yǎng)板)
    UF-4001L-Case(每箱40塊)UF-4001L-Each(每塊)UniFlex Culture Plate-Laminin (6孔層粘連蛋白UniFlex™單向應(yīng)力細(xì)胞培養(yǎng)板)
    3、HT BIOFLEX®CULTURE PLATES(高通量BIOFLEX®培養(yǎng)板培養(yǎng)板)
    白色板

    編號(hào)產(chǎn)品

    產(chǎn)品名稱

    HTPW-3001U-Case(每箱40塊)HTPW-3001U-Each(每塊)White HT BioFlex Culture Plate-Untreated(白色24孔高通量表面未處理培養(yǎng)板)
    HTPW-3001A-Case(每箱40塊)HTPW-3001A-Each(每塊)White HT BioFlex Culture Plate-Amino(白色24孔高通量氨基培養(yǎng)板)
    HTPW-3001C-Case(每箱40塊)HTPW-3001C-Each(每塊)White HT BioFlex Culture Plate-Collagen Type I(白色24孔高通量膠原I型培養(yǎng)板)
    HTPW-3001C(IV)-Case(每箱40塊)HTPW-3001C(IV)-Each(每塊)White HT BioFlex Culture Plate-Collagen Type IV(白色24孔高通量膠原IV型培養(yǎng)板)
    HTPW-3001E-Case(每箱40塊)HTPW-3001E-Each(每塊)White HT BioFlex Culture Plate-Elastin(白色24孔高通量彈力培養(yǎng)板)
    HTPW-3001P-Case(每箱40塊)HTPW-3001P-Each(每塊)White HT BioFlex Culture Plate-ProNectin(白色24孔高通量ProNectin 培養(yǎng)板)
    HTPW-3001L-Case(每箱40塊)HTPW-3001L-Each(每塊)White HT BioFlex Culture Plate-Laminin(白色24孔高通量層粘連蛋白培養(yǎng)板)
    黑色板
    HTPB-3001U-Case(每箱40塊)HTPB-3001U-Each(每塊)Black HT BioFlex Culture Plate-Untreated(黑色24孔高通量表面未處理培養(yǎng)板)
    HTPB-3001A-Case(每箱40塊)HTPB-3001A-Each(每塊)Black HT BioFlex Culture Plate-Amino(黑色24孔高通量氨基培養(yǎng)板)
    HTPB-3001C-Case(每箱40塊)HTPB-3001C-Each(每塊)Black HT BioFlex Culture Plate-Collagen Type I(黑色24孔高通量膠原I培養(yǎng)板)
    HTPB-3001C(IV)-Case(每箱40塊)HTPB-3001C(IV)-Each(每塊)Black HT BioFlex Culture Plate-Collagen Type IV(黑色24孔高通量膠原IV培養(yǎng)板)
    HTPB-3001E-Case(每箱40塊)HTPB-3001E-Each(每塊)Black HT BioFlex Culture Plate-Elastin(黑色24孔高通量彈力培養(yǎng)板)
    HTPB-3001P-Case(每箱40塊)HTPB-3001P-Each(每塊)Black HT BioFlex Culture Plate-ProNectin(黑色24孔高通量ProNectin培養(yǎng)板)
    HTPB-3001L-Case(每箱40塊)HTPB-3001L-Each(每塊)Black HT BioFlex Culture Plate-Laminin(黑色24孔高通量層粘連蛋白培養(yǎng)板)
    該FX-5000T系統(tǒng)增配的三維培養(yǎng)功能模塊后三維細(xì)胞組織培養(yǎng)配套耗材
    CIRCULAR FOAM TISSUE TRAIN CULTURE PLATES
    (圓形三維細(xì)胞組織培養(yǎng)板采用彈性底部,可用來制備三維基質(zhì)蛋白細(xì)胞培養(yǎng)物,并提供雙軸向拉力,不需要生物膠槽(Trough Loader))
    編號(hào)產(chǎn)品產(chǎn)品名稱
    TTCF-4001U-CaseTTCF-4001U-EachCircular Foam Culture Plate-Untreated
    TTCF-4001A-CaseTTCF-4001A-EachCircular Foam Culture Plate-Amino
    TTCF-4001C-CaseTTCF-4001C-EachCircular Foam Culture Plate-Collagen Type I
    TTCF-4001C(IV)-CaseTTCF-4001C(IV)-EachCircular Foam Culture Plate-Collagen Type IV
    TTCF-4001E-CaseTTCF-4001E-EachCircular Foam Culture Plate-Elastin
    TTCF-4001P-CaseTTCF-4001P-EachCircular Foam Culture Plate-ProNectin
    TTCF-4001L-CaseTTCF-4001L-EachCircular Foam Culture Plate-Laminin
    TISSUE TRAIN CULTURE PLATES
    (三維細(xì)胞組織培養(yǎng)板采用彈性底部,可用來制備三維基質(zhì)蛋白細(xì)胞培養(yǎng)物,并提供單軸向拉力。)
    編號(hào)產(chǎn)品產(chǎn)品名稱
    Foruse with Standard Trough Loaders (與線形生物膠槽配套使用)
    TT-4001U-CaseTT-4001U-EachTissue Train Culture Plate-Untreated
    TT-4001A-CaseTT-4001A-EachTissue Train Culture Plate-Amino
    TT-4001C-CaseTT-4001C-EachTissue Train Culture Plate-Collagen Type I
    TT-4001C(IV)-CaseTT-4001C(IV)-EachTissue Train Culture Plate-Collagen Type IV
    TT-4001E-CaseTT-4001E-EachTissue Train Culture Plate-Elastin
    TT-4001P-CaseTT-4001P-EachTissue Train Culture Plate-ProNectin
    TT-4001L-CaseTT-4001L-EachTissue Train Culture Plate-Laminin
    Foruse with Trapezoidal Trough Loaders (與梯形生物膠槽配套使用) 
    編號(hào)產(chǎn)品產(chǎn)品名稱
    TTTP-4001U-CaseTTTP-4001U-EachTrapezoidal TT Culture Plate-Untreated
    TTTP-4001A-CaseTTTP-4001A-EachTrapezoidal TT Culture Plate-Amino
    TTTP-4001C-CaseTTTP-4001C-EachTrapezoidal TT Culture Plate-Collagen Type I
    TTTP-4001C(IV)-CaseTTTP-4001C(IV)-EachTrapezoidal TT Culture Plate-Collagen Type IV
    TTTP-4001E-CaseTTTP-4001E-EachTrapezoidal TT Culture Plate-Elastin
    TTTP-4001P-CaseTTTP-4001P-EachTrapezoidal TT Culture Plate-ProNectin
    TTTP-4001L-CaseTTTP-4001L-EachTrapezoidal TT Culture Plate-Laminin
    該FX-5000T系統(tǒng)增配的壓應(yīng)力加載功能模塊后三維細(xì)胞組織壓力培養(yǎng)配套耗材
    CIRCULAR FOAM TISSUE TRAIN CULTURE PLATES
    (圓形三維細(xì)胞組織培養(yǎng)板采用彈性底部,可用來制備三維基質(zhì)蛋白細(xì)胞培養(yǎng)物,并提供雙軸向拉力,不需要生物膠槽(Trough Loader))
    編號(hào)產(chǎn)品產(chǎn)品名稱
    TTCF-4001U-CaseTTCF-4001U-EachCircular Foam Culture Plate-Untreated

 

BioFlex® Culture Plate 
Catalog Page   Product Information Brochure Application Page


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Flexible-bottomed culture plate that works with the Flexcell® FX-5000™ Tension Systemto provide a mechanical load regimen to cells in monolayer (see Fig. 1 below).
  • 6-well culture plate with total growth surface area of 57.75 cm2.
  • Rubber membranes are optically clear for direct viewing of cells with an inverted or upright microscope.
  • Covalently bound matrix surfaces: Amino, Collagen (Type I or IV), Elastin, Pronectin® (RGD), and Laminin (YIGSR). Learn more in Tech Report 106: Matrix Bonded Growth Surfaces. Growing Cells in a More Natural Matrix Environment.
  • Available individually or by the case of 40* plates.
*5% Savings (based on buying 40 plates at the individual price)


Applications of BioFlex® Culture Plates
  • Mechanical Load - use with the FX-5000™ Tension System to research the effects of equibiaxial tensile strain on cells in monolayer culture.
  • Softer Substrate - provides a softer substrate for cell growth (when compared to standard plastic culture dishes) which better mimics in situ conditions for some cell types like trabecular meshwork cells (see Aga et al. Invest Ophthalmol Vis Sci 55(9):5497-509, 2014).
  • Matrix Coated Surface - culture plate surfaces are coated with various matrix proteins, such as type I collagen and laminin, to better simulate in vivo conditions (see Tech Report 106: Matrix Bonded Growth Surfaces. Growing Cells in a More Natural Matrix Environment).
  • Traumatic Injury - can be used in studies looking at traumatic injury in astrocytes and neurons, where the injury is produced with a Cell Injury Controller that controls a burst of gas which deforms the BioFlex® membrane creating large biaxial strains (see Ellis et al. J Neurotrauma 12 325–339, 1995Ahmed et al. J Neurochem 74(5):1951-1960, 2000Augustine et al. Neuroscience 274:1-10, 2014).
Read more about Applying Mechanical Load to Cells in Monolayer Culture
 
Equibiaxial Strain

Figure 1: Equibiaxial strain application (with a FX-5000™ Tension System) to cells plated in the well of a BioFlex® culture plate.

Relevant Tech Reports & Other Information
  • 101: Loading Stations™. Quantification of Strain on the Membrane Surface
  • 106: Matrix Bonded Growth Surfaces. Growing Cells in a More Natural Matrix Environment
  • 202: Viewing Cells on Flexcell®'s Culture Plates. Light and Electron Microscope Techniques and Immunofluorescent Imaging 
     
  • BioFlex® Culture Plates Product Information
  • 6-Well Equibiaxial Loading Stations™ Product Information
  • FlexStop™ Product Information
  • Flexcell® Transwell Holder Product Information 
     
  • Culture Plate & Loading Station™ User's Manual
  • Flexcell® FX-5000™ Tension System User Manual
  •  

 

 

The History of Flexcell®
In 1978, Albert J. Banes, Ph.D., an Assistant Professor in the Surgery Department at the University of North Carolina at Chapel Hill, received his first small grant for devising a method to apply regulated strain to cultured cells. He built prototypes of the current Flexcell® Tension System in the shop using only a timer, a simple solenoid valve to control pressure in on/off modes, and a needle valve to regulate the magnitude of the pressure. He built a baseplate with vacuum channels in Plexiglas and used natural gum rubber as the seal between the culture plate bottom and the vacuum chamber. Dr. Banes polymerized his own rubber membranes from medical grade Dow silastic components. A cast material that eventually proved satisfactory for the membrane in a 6-well, 35 mm diameter culture plate that had the well bottoms drilled out to receive the cast membranes. A Commodore Vic 20 was the first commercial microprocessor used to control the valves. The software program and a controller board were designed by Dr. Banes and a well grounded electronics engineer, Olivier Monbureau. A Commodore 64 was the next generation computer used, followed by a Tandy computer, then IBM clones. Today, Flexcell® develops its own software and builds custom computers and controller boards and assembles them at its plant in Burlington, NC.

Dr. Banes' inlectual property was awarded the first patent in the field of Cytomechanics. He believed that this technology could become important scientifically as well as medically if a market driver could be found. In his first patent describing the technology, he included the thought that cyclically stretching cells and tissues could lead to adaptation of cells to a mechanically active environment such as dermal fibroblasts cultured in vitro in a skin construct. Along with his father in Pennsylvania, G.B. Banes, J.D., they created Flexcell® Inc. The company's mission was to commercialize the technology to make it available to all scientists as well as find medically relevant uses. His first full publication (Journal of Cell Science, 1985) in the area demonstrated the utility of the system and that cyclic strain altered the expression of cytoplasmic filament proteins such as actin and tubulin in tendon cells.

Continued development of the technologies behind the Flexcell® Tension and Compression Systems has taken much of Dr. Banes' time. Dr. Banes became the first university professor to be permitted to retain his academic professorship and be president of a company (Flexcell® International Corporation) by a decision of the Committee on Industrial Faculty Relations at the University of North Carolina at Chapel Hill.

Dr. Banes holds three patents on the basic cell stretching technology and several more on other technologies including a DNA transfection technique using flexation of cells and a spill resistant culture plate called the SpillGuard® culture plate. He continues to develop new products for cell culture and cell stretching as well as submit patents assigned to Flexcell® International Corporation.
 

 


Our Mission

  • To be an industry leading developer and manufacturer of biotechnology products for cellular and tissue biomechanics research.
  • To provide innovative products and solutions to researchers enabling the advancement of molecular biology and tissue engineering research.
  • To be a valued business partner to both our suppliers and distributors and honorable employer to our workforce while maintaining a profitable operation.


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