Product Description

This pan-specific anti-acetyllysine antibody is from a mouse monoclone hybridoma and is affinity purified using acetyllysine–specific affinity chromatography. It recognizes both of free acetyllysine and acetyllysine as residues in a protein or peptides. The product has been utilized for proteomic studies of the acetylated protein, immunoaffinity chromatography separation and isolation of the acetylated protein and peptides from protease-digesting proteins of whole cells.

A           B

Chicken erythrocytes histones were separated by Triton-Acetic Acid-Urea (1) non-treated and(2) Sodium butyrate (5 mM) treated. Each lane contained 20 ug of total protein. PVDF membrane was probed with mouse monoclonalantibody (ICP0390 was detected with a goat anti-mouse HRPO secondary antibody (1:50000) and developed by chemiluminescence.

Species

Mouse

Formulation

250 μg/mL antibody stored in PBS, 50% glycerol

Immunogen

Acetylated KLH conjugates

Purification

The antibody was purified on acetyl-lysine agarose.

Specificity

The antibody recognizes proteins acetylated on lysine residues. Tested: acetylated histone, acetylated BSA, and acetylated MBP. There are no reactions to non-acetylated proteins.

Applications

ELISA; WB (1:1000 - 1:3000); IP (approximay 10 ug/mg crude protein)

Scientific Description

DNA transcription cannot take place unless DNA is unwound from the nucleosomes. The cell unwinds the DNA by acetylating lysine residues within the proteins. It has been suggested that acetylation of non-histone proteins (e.g., transcription factors) and histones may be involved. Acetylation of these proteins may result in signal transduction within the chromatic domains.

Storage & Stability

Product is stable for several weeks at 4 C. For extended storage, store product at –20ºC. Expiration date is 12 months from date of shipping if properly stored.