Dispenser pump tubing Kit. Basic arrangement Celartia Transforming the art of cell culture in scientific engineering www.celartia。。ｃｏｍ RESERVOIR RESERVOIR * The 0.2 microns pore air filter allows for emptying the tubing (recover the media) in sterile conditions with the pump in reverse flow

PETAKA G3低氧高氧細胞培養(yǎng)板，缺氧缺氧培養(yǎng)板，細胞培養(yǎng)技術革新 --無需提供CO2的高仿真體外培養(yǎng)系統(tǒng)！獲得美國、歐洲廣大生物科學家的青睞！

1. 的Micro-tubular gas diffusion channel，保證培養(yǎng)基中氧氣的濃度；
2. 無需CO2和濕度培養(yǎng)箱，嚴格模擬體內生長環(huán)境；
3. 可雙面培養(yǎng)，培養(yǎng)方式可豎直或水平培養(yǎng)；
4. 可根據(jù)培養(yǎng)細胞的特性，根據(jù)細胞的耗氧情況，選擇適應于相應細胞的培養(yǎng)條件；
5. 高密閉性，無開放的加樣口、排氣口；
6. 可直接在6×，10×，20×，25×顯微鏡下進行細胞觀察；
7. 操作簡單，易掌握；
8. 功能多樣；一板多用；
9. 細胞培養(yǎng)——密閉性好；無菌性高；高仿真的體內模擬環(huán)境；
10. 細胞休眠——細胞運輸；短期保存；細胞周期和凋亡研究；
11. 細胞凍存——無需額外的凍存管和凍存盒；條形碼，便于管理；凍存細胞數(shù)量遠遠大于凍存管；凍存液使用量少；直接觀察凍存細胞形態(tài)；冷凍速度快；復蘇快，操作簡單；
12. 細胞運輸——成本低；減少細胞轉移次數(shù)和活化操作；不易污染；
13. 細胞分選——免疫學研究&臨床診斷；細胞傳染后的分選；細胞生理學研究和病理學研究；干細胞研究和再生；骨髓干細胞分選收集臍帶血干細胞；
14. 無細胞損傷的收集——省時省力；無細胞損傷；用于流式細胞分析；細胞表面的電鏡觀察；
15. 細胞直接觀察

Petaka培養(yǎng)系統(tǒng)不需要CO2培養(yǎng)箱或者濕度培養(yǎng)箱，僅在合適的培養(yǎng)溫度下即可生長，使得培養(yǎng)環(huán)境簡易化；根據(jù)細胞的特性，可靈活選擇合適的含氧條件（低氧/高氧），模擬體內培養(yǎng)環(huán)境；密閉式加樣口和排氣口，保證了培養(yǎng)環(huán)境的封閉性，減少污染幾率。

Petaka 細胞培養(yǎng)系統(tǒng)中Micro-tubular gas diffusion channel，是Celartia公司的技術，自動平衡培養(yǎng)基質中的溶解氧含量——45mmHg，嚴格模擬細胞的天然生長條件。同時Petaka 細胞培養(yǎng)系統(tǒng)可根據(jù)培養(yǎng)細胞的類型，耗氧情況，從而減少培養(yǎng)系統(tǒng)中的氧氣含量，比如常規(guī)的21%的氧氣含量降低至5%或2%。

觀點一： 京都大學教授山中伸彌等人在iPS細胞研究過程中，發(fā)現(xiàn)機體內的干細胞總是集中于氧氣相對少的地方。于是，他們在利用人體皮膚細胞培養(yǎng)iPS細胞時把培養(yǎng)環(huán)境的氧濃度從通常的21％降到5％，發(fā)現(xiàn)iPS細胞的生成效率可提高到原來的2．5倍至4．2倍。

觀點二： 低氧是生物體常見的一種生理和病理現(xiàn)象，不同組織的細胞生長在不同的氧濃度下，在探討氧環(huán)境是否影響相關干細胞分化上值得我們干細胞工作者們做深入研究，尤其是在胚胎發(fā)育過程中氧濃度的變化對相關組織的形成與發(fā)育研究在不久的將來定會有重大的突破。

觀點三： 低氧環(huán)境對胚胎早期發(fā)育過程影響很大，像體外培養(yǎng)的細胞（常壓/常氧），與體內相比，表觀修飾發(fā)生了巨大變化。

PetakaG3™ LOT (Low Oxygen Transfer)

The cell culture device PetakaG3 is an innovative bioreactor designed for mammalian cell culture in physiologic tissue conditions. The distinctive feature of PetakaG3 is that being a disposable device incorporates an automatic gas diffusion control system (AGD-CS) that works interactively with the cells growing and living inside, WITHOUT HYPOXIA CAMBERS, GLOVE BOXES OR THREE-GAS INCUBATORS

For cells cultured in PetakaG3 and incubated in regular 21% oxygen atmosphere the AGD-CS provides:

1) Media dissolved oxygen concentration within the limits of the physiologic oxygen tensions in physiologic tissues (from 75 mmHg down to 15 mmHg) monitorable with a dissolved oxygen fluorescent sensor dot. Because this advanced trait PetakaG3 is the safest, smallest, unique portable disposable hypoxia chamber, also available with integrated DO sensor.

Average DO = 1 mg/L (0.5 to 1.1 mg/L depending on cell type), equivalent to average 22.7 mmHg (Torr) in tissues or equivalent to average 3.11 %O2 in hypoxia chambers.

                  PDF. Additional information

2) Media dissolved CO2 concentration, enough to maintain a physiologic pH without external extra-supply of carbon dioxide gas.

3) Controlled media evaporation even at 2% to 10% atmospheric Relative Humidity (RH).

PetakaG3 allows cultures of two different cell types in the same device. Both cell layers are separated by 3mm of media, which is appropriate to study intercellular interactions through diffusible factors and exosome exchange. PetakaG3 is ideal for transfection of both cell layers with two different gene constructs in the same device with negligible cross transfection

Global specifications of PetakaG3 LOT:

1. It acts as an independent hypoxia chamber between 1% and 21% Oxygen. Medium Dissolved Oxygen auto-controlled (O2PP  between 2 and 50 mmHg)
2. No additional CO₂ required for any type of media, although the PetakaG3™device can also be used in CO₂ incubators

3. Humidity autocontrolled, no added humidity required (not influenced by environment)

4. The most secure cell culture device against contamination (no caps, no openings)
5. Designed to transport live cells without requiring freezing and dry ice
6. Optimizes media and growth factor supplies
7. Can provide up to 15 times more cell culture surface area per incubator capacity
8. Both surfaces may be viewed using any type of light microscope
9. In vitro cell pause or dormancy is easily accomplished
10. Allows storage of pre-filled units that are ready to host cells
11. Easy to handle. It operates in any position. Compley safe & versatile
12. Each Petaka is individually bar coded for easy tracking.

PetakaG3™ HOT (High Oxygen Transfer)

The cell culture device Petaka G3 with orange color port.

PetakaG3™ HOT bioreactor is manufactured from a special co-polymer which has a particularly high diffusion rate of oxygen and carbon dioxide through the walls of the device.

Average DO  = 3 mg/L (2.8 to 3.2 mg/L depending on cell type), equivalent to 66.5 mmHg in tissues or equivalent to 9.33 %O2 in hypoxia chambers.

Because of its high diffusion coefficients requires to be incubated in 5% CO2 with saturated relative humidity to avoid dehydration.

PetakaG3™ HOT has a gas self regulating system that maintains the level of dissolved oxygen in the cell culture media like in all Petaka G3, but the minimal level that can reach is higher, for a given cell type and cell density. Example: in the same cell culture conditions (cell type, cell density and media type) when Petaka G3 balances the oxygen exchange at 1ppm, Petaka G3 HOT will hold it at 3ppm, maintaining the median level of dissolved oxygen in the highest limit of the physiologic oxygen tension of the tissues, equivalent to 10% concentration of oxygen in the open atmosphere.
 
PetakaG3™ HOT bioreactor is particularly suited for culturing cells with a high aerobic metabolism, for example primary liver cell cultures, hematopoietic bone marrow cells, well differentiate keratinocytes, hybridomas, Pancreatic islets, etc.  

Cells cultured for a long time in traditional flasks and dishes, exposed to 21% oxygen of open atmosphere, are metabolically adapted to such environment, far from the in vivo reality and behavior. 

PetakaG3™ FLAT (Suspended Cell Culture)

 Special applcations Cell culture devices.-

The PetakaG3™ FLAT bioreactor (For Low ATachmet) has all of the same size and structure characteristics as the PetakaG3™ either LOT or HOT bioreactor, except that the interior surfaces are not treated to promote cell adherence to its walls.This special bioreactor is produced with a flat polymer with a very low surface tension.

Applications:

Suspended cell cultures such as; Blood cell preparation, Leukemia Cells, Hybridomas Cells, Lymphocyte culture and activation for kariotyping.

Special cells necessary for the bio-synthesis of specific products for pharmaceutical applications.

Cultures of cells with self agregation such as spheroids, 3D cultures, pseudo-tissues, etc.

PetakaG3™ ET™ (Easy Transfer)

The cell culture device PetakaG3™ ET™ bioreactor is a PetakaG3™ that includes inside the cell culture chamber a set of free steel doped micro-bars (stirrer bars) that can be moved by magnetic fields.

The Petaka™ ET bioreactor is used in conjunction with either an automatic magnetic random scanner (Cell Flipper) that drags the stir bars across the internal surface of Petaka or with a manual magnetic tablet (Cell lifter) on which the user drags the Petaka to detach the cells.

The turbulences created by the stir bar motion over the cells are strong enough to induce cell enzyme free cell detachment without the need for chemicals. Depending on cell type the automatic scanning is fast for cell harvesting and preserves a high yield of unharmed detached cells that can be directly transferred to new bioreactors without washing. 

The manual Cell Lifter is used for detaching fragile cells carefully; the operator controls the speed and the specific areas of the cell monolayer to be detached.

Petaka G3 ET is ideal for enzyme free detachment for any kind of cell line, and extremely well suited for Stem cell cultures, also allows for safe and frequent cell culture samplings during growth, and in vitro evaluation of wound healing.

PetakaG3™ MATRIX (Specific Molecular Matrix)

The cell culture device Petaka G3 MATRIX could be LOT or HOT

The PetakaG3™ MATRIX bioreactor has all of the same size and structure characteristics as the PetakaG3™  bioreactor. 

PetakaG3™ MATRIX is Coated with Specific Molecular Matrix. These are several types of Petaka internally coated with specific molecular matrix incluiding collagen, fibronectin, poly-L-lysine, matrigel, gelatin, laminin.

This product is usually best suitable for growing different types of stem cells.

Because of the specialty matrices involved, these bioreactors are only available on a made-to-order basis.