PETAKA G3低氧高氧細(xì)胞培養(yǎng)板，缺氧缺氧培養(yǎng)板，細(xì)胞培養(yǎng)技術(shù)革新 --無(wú)需提供CO2的高仿真體外培養(yǎng)系統(tǒng)！獲得美國(guó)、歐洲廣大生物科學(xué)家的青睞！

1. 的Micro-tubular gas diffusion channel，保證培養(yǎng)基中氧氣的濃度；
2. 無(wú)需CO2和濕度培養(yǎng)箱，嚴(yán)格模擬體內(nèi)生長(zhǎng)環(huán)境；
3. 可雙面培養(yǎng)，培養(yǎng)方式可豎直或水平培養(yǎng)；
4. 可根據(jù)培養(yǎng)細(xì)胞的特性，根據(jù)細(xì)胞的耗氧情況，選擇適應(yīng)于相應(yīng)細(xì)胞的培養(yǎng)條件；
5. 高密閉性，無(wú)開(kāi)放的加樣口、排氣口；
6. 可直接在6×，10×，20×，25×顯微鏡下進(jìn)行細(xì)胞觀(guān)察；
7. 操作簡(jiǎn)單，易掌握；
8. 功能多樣；一板多用；
9. 細(xì)胞培養(yǎng)——密閉性好；無(wú)菌性高；高仿真的體內(nèi)模擬環(huán)境；
10. 細(xì)胞休眠——細(xì)胞運(yùn)輸；短期保存；細(xì)胞周期和凋亡研究；
11. 細(xì)胞凍存——無(wú)需額外的凍存管和凍存盒；條形碼，便于管理；凍存細(xì)胞數(shù)量遠(yuǎn)遠(yuǎn)大于凍存管；凍存液使用量少；直接觀(guān)察凍存細(xì)胞形態(tài)；冷凍速度快；復(fù)蘇快，操作簡(jiǎn)單；
12. 細(xì)胞運(yùn)輸——成本低；減少細(xì)胞轉(zhuǎn)移次數(shù)和活化操作；不易污染；
13. 細(xì)胞分選——免疫學(xué)研究&臨床診斷；細(xì)胞傳染后的分選；細(xì)胞生理學(xué)研究和病理學(xué)研究；干細(xì)胞研究和再生；骨髓干細(xì)胞分選收集臍帶血干細(xì)胞；
14. 無(wú)細(xì)胞損傷的收集——省時(shí)省力；無(wú)細(xì)胞損傷；用于流式細(xì)胞分析；細(xì)胞表面的電鏡觀(guān)察；
15. 細(xì)胞直接觀(guān)察

Petaka培養(yǎng)系統(tǒng)不需要CO2培養(yǎng)箱或者濕度培養(yǎng)箱，僅在合適的培養(yǎng)溫度下即可生長(zhǎng)，使得培養(yǎng)環(huán)境簡(jiǎn)易化；根據(jù)細(xì)胞的特性，可靈活選擇合適的含氧條件（低氧/高氧），模擬體內(nèi)培養(yǎng)環(huán)境；密閉式加樣口和排氣口，保證了培養(yǎng)環(huán)境的封閉性，減少污染幾率。

Petaka 細(xì)胞培養(yǎng)系統(tǒng)中Micro-tubular gas diffusion channel，是Celartia公司的技術(shù)，自動(dòng)平衡培養(yǎng)基質(zhì)中的溶解氧含量——45mmHg，嚴(yán)格模擬細(xì)胞的天然生長(zhǎng)條件。同時(shí)Petaka 細(xì)胞培養(yǎng)系統(tǒng)可根據(jù)培養(yǎng)細(xì)胞的類(lèi)型，耗氧情況，從而減少培養(yǎng)系統(tǒng)中的氧氣含量，比如常規(guī)的21%的氧氣含量降低至5%或2%。

觀(guān)點(diǎn)一： 京都大學(xué)教授山中伸彌等人在iPS細(xì)胞研究過(guò)程中，發(fā)現(xiàn)機(jī)體內(nèi)的干細(xì)胞總是集中于氧氣相對(duì)少的地方。于是，他們?cè)诶萌梭w皮膚細(xì)胞培養(yǎng)iPS細(xì)胞時(shí)把培養(yǎng)環(huán)境的氧濃度從通常的21％降到5％，發(fā)現(xiàn)iPS細(xì)胞的生成效率可提高到原來(lái)的2．5倍至4．2倍。

觀(guān)點(diǎn)二： 低氧是生物體常見(jiàn)的一種生理和病理現(xiàn)象，不同組織的細(xì)胞生長(zhǎng)在不同的氧濃度下，在探討氧環(huán)境是否影響相關(guān)干細(xì)胞分化上值得我們干細(xì)胞工作者們做深入研究，尤其是在胚胎發(fā)育過(guò)程中氧濃度的變化對(duì)相關(guān)組織的形成與發(fā)育研究在不久的將來(lái)定會(huì)有重大的突破。

觀(guān)點(diǎn)三： 低氧環(huán)境對(duì)胚胎早期發(fā)育過(guò)程影響很大，像體外培養(yǎng)的細(xì)胞（常壓/常氧），與體內(nèi)相比，表觀(guān)修飾發(fā)生了巨大變化。

PetakaG3™ LOT (Low Oxygen Transfer)

The cell culture device PetakaG3 is an innovative bioreactor designed for mammalian cell culture in physiologic tissue conditions. The distinctive feature of PetakaG3 is that being a disposable device incorporates an automatic gas diffusion control system (AGD-CS) that works interactively with the cells growing and living inside, WITHOUT HYPOXIA CAMBERS, GLOVE BOXES OR THREE-GAS INCUBATORS

For cells cultured in PetakaG3 and incubated in regular 21% oxygen atmosphere the AGD-CS provides:

1) Media dissolved oxygen concentration within the limits of the physiologic oxygen tensions in physiologic tissues (from 75 mmHg down to 15 mmHg) monitorable with a dissolved oxygen fluorescent sensor dot. Because this advanced trait PetakaG3 is the safest, smallest, unique portable disposable hypoxia chamber, also available with integrated DO sensor.

Average DO = 1 mg/L (0.5 to 1.1 mg/L depending on cell type), equivalent to average 22.7 mmHg (Torr) in tissues or equivalent to average 3.11 %O2 in hypoxia chambers.

                  PDF. Additional information

2) Media dissolved CO2 concentration, enough to maintain a physiologic pH without external extra-supply of carbon dioxide gas.

3) Controlled media evaporation even at 2% to 10% atmospheric Relative Humidity (RH).

PetakaG3 allows cultures of two different cell types in the same device. Both cell layers are separated by 3mm of media, which is appropriate to study intercellular interactions through diffusible factors and exosome exchange. PetakaG3 is ideal for transfection of both cell layers with two different gene constructs in the same device with negligible cross transfection

Global specifications of PetakaG3 LOT:

1. It acts as an independent hypoxia chamber between 1% and 21% Oxygen. Medium Dissolved Oxygen auto-controlled (O2PP  between 2 and 50 mmHg)
2. No additional CO₂ required for any type of media, although the PetakaG3™device can also be used in CO₂ incubators

3. Humidity autocontrolled, no added humidity required (not influenced by environment)

4. The most secure cell culture device against contamination (no caps, no openings)
5. Designed to transport live cells without requiring freezing and dry ice
6. Optimizes media and growth factor supplies
7. Can provide up to 15 times more cell culture surface area per incubator capacity
8. Both surfaces may be viewed using any type of light microscope
9. In vitro cell pause or dormancy is easily accomplished
10. Allows storage of pre-filled units that are ready to host cells
11. Easy to handle. It operates in any position. Compley safe & versatile
12. Each Petaka is individually bar coded for easy tracking.