RF-O2熒光光纖氧氣測量技術(shù)——氧氣測量全面解決方案

    RF-O2熒光光纖氧氣測量技術(shù)是基于REDFLASH光極傳感器技術(shù)的氧氣測量技術(shù)，由歐洲Pyroscience公司及Graz大學(xué)等科學(xué)家研制生產(chǎn)，由光極氧氣傳感器、測量儀及軟件組成，廣泛應(yīng)用于環(huán)境科學(xué)、生態(tài)科學(xué)、植物科學(xué)、動物科學(xué)、海洋科學(xué)、生物醫(yī)學(xué)、生物技術(shù)、食品科學(xué)等各個領(lǐng)域，其主要功能特點如下

1. REDFLASH光極氧氣傳感器技術(shù)，高精確度、高穩(wěn)定性、高時空解析度、低能耗、無耗氧、無交叉敏感性
2. 傳感器類型靈活多樣，有探頭式、探針式、非接觸式（sensor spot）及納米微粒式等，適應(yīng)于液體和氣體不同條件下的O2測量
3. 有內(nèi)置sensor spot的流通管和呼吸瓶，非接觸式測量流動液體的溶解氧及呼吸瓶內(nèi)液體或氣體中氧氣含量
4. 輕便緊湊型FireStingO2測量儀，內(nèi)置水汽、氣壓傳感器，有1、2、4通道供選配，可分別接1個、2個、4個光極氧氣傳感器，另有Mini型FireStingO2-mini供選配
5. U盤式PiccolO2測量儀——世界上zui小的O2測量儀，可連接一個O2傳感器，USB口連接電腦，即插即用

測量原理：

    REDFLASH光極O2傳感器技術(shù)，利用*的O2敏感REDFLASH指示劑，通過610-630nm調(diào)制紅光激發(fā)，REDFLASH指示劑發(fā)出760-790nm紅外熒光，熒光強度隨接觸的O2分子濃度升高而發(fā)生熒光淬滅，這種熒光動態(tài)通過光纖傳輸?shù)綔y量儀，測量儀靈敏地檢測其相位漂移并據(jù)此換算成O2濃度

應(yīng)用領(lǐng)域：

1. 水體溶解氧測量監(jiān)測、藻類及藻類生物膜光合作用與呼吸作用測量監(jiān)測
2. 植物光合作用與呼吸作用測量監(jiān)測
3. 水生動物（魚類、水生昆蟲等無脊椎動物、浮游動物等呼吸代謝測量
4. 陸生動物、實驗動物、動物組織、血液等呼吸代謝測量
5. 土壤、濕地、海洋沉積、河湖沉積剖面O2測量
6. 生物反應(yīng)器、發(fā)酵過程、酶動力學(xué)、細胞培養(yǎng)等O2測量監(jiān)測
7. 糧食食品儲運、葡萄酒等O2測量監(jiān)測
8. 污水處理、沼氣、垃圾填埋場、有機物降解等O2測量監(jiān)測

技術(shù)指標(biāo)：

1. FireStingO2（FSO2）測量儀：
   1. 有1通道、2通道、4通道可供選配，分別可接1個、2個和4個O2傳感器，可并聯(lián)組成8通道甚至更多通道；另具備一個溫度傳感器通道（可選配4通道溫度傳感器）
   2. 激發(fā)光源620nm，監(jiān)測器760nm（NIR）
   3. 采樣頻率：每秒4次
   4. 內(nèi)置氣壓傳感器，300－1100mbar，0.06mbar分辨率，精確度±3mbar
   5. 內(nèi)置濕度傳感器，0－100%，分辨率0.04%，精確度±0.2%
   6. 內(nèi)置溫度傳感器，-40－125°C，分辨率0.01°C，精確度±0.3°C
   7. 具模擬輸出和自動模式，0－2.5VDC
   8. USB接口，通過USB口PC供電
   9. 大小：68x120x30mm，重350g
2. PiccolO2 U盤式測量儀：大小僅15x15x54mm，重量約20g，單通道，激發(fā)光620nm，檢測器760nm，采樣頻率每秒20次?？刹⒙?lián)組成多通道測量系統(tǒng)?？赏ㄟ^PiccoTHP測量溫濕度和氣壓并進行補償

1. 探頭式O2傳感器：直徑3mm，測量范圍0-50%（0-23mg/l）（可選配其它范圍），檢測極限0.02%（0.01mg/l），分辨率0.05%（0.025mg/l）@20% O2，精確度±0.2％（0.1mg/l）@20% O2，zui低使用壽命1千萬數(shù)據(jù)點，存儲時間大于3年（室溫暗處儲放）
2. 探針式O2傳感器：有固定探針式、可伸縮探針式、尖頭式及圓頭式等不同類型供選配；探針直徑有50μm、230μm、430μm等規(guī)格；測量范圍0-50%（0-23mg/l）（可選配其它范圍），檢測極限0.02%（0.01mg/l），分辨率0.05%（0.025mg/l）@20% O2，精確度±0.2％（0.1mg/l）@20% O2，zui快響應(yīng)時間小于1s（與探針粗細有關(guān)），zui低使用壽命1百萬數(shù)據(jù)點，存儲時間大于3年（室溫暗處儲放）

1. 非接觸式（sensor spot）O2傳感器（見下左圖）：用于非接觸性測量監(jiān)測透明容器中的氧氣含量，傳感器貼用硅膠等貼附在容器內(nèi)壁，通過固定在外壁的光纖將熒光動態(tài)信號傳輸?shù)綔y量儀以檢測O2濃度；測量范圍0-50%（0-23mg/l）（可選配其它范圍），檢測極限0.02%（0.01mg/l），分辨率0.05%（0.025mg/l）@20% O2，精確度±0.2％（0.1mg/l）@20% O2，zui低使用壽命2千萬數(shù)據(jù)點，存儲時間大于3年（室溫暗處儲放）

1. 納米微粒傳感器（參見上右圖）：納米技術(shù)，用于非接觸性測量微量液體中O2含量，即時響應(yīng)，測量范圍0-50%（0-23mg/l），檢測極限0.02%（0.01mg/l），分辨率0.05%（0.025mg/l）@20% O2，存儲時間大于3年（室溫暗處儲放）
2. 流通管：內(nèi)置非接觸式O2傳感器，用于流動液體O2測量監(jiān)測（如魚類呼吸代謝測量等），測量范圍0-50%（0-23mg/l）（可選配其它范圍），檢測極限0.02%（0.01mg/l），分辨率0.05%（0.025mg/l）@20% O2，精確度±0.2％（0.1mg/l）@20% O2，zui低使用壽命1千萬數(shù)據(jù)點，存儲時間大于3年（室溫暗處儲放）
3. 呼吸瓶：內(nèi)置非接觸式O2傳感器，用于生物呼吸測量（如藻類、小型魚類、魚卵、昆蟲等），標(biāo)準(zhǔn)配置有4ml和20ml兩種規(guī)格
4. Pyro Oxygen Logger軟件用于參數(shù)設(shè)置、校準(zhǔn)、數(shù)據(jù)顯示包括圖表顯示、數(shù)據(jù)輸出等功能

應(yīng)用案例：

案例1：法國Bordeaux大學(xué)利用FSO2 4通道熒光光纖氧氣測量儀，對Aquitaine海岸沉積樣芯耗氧進行了測量分析，以研究海洋底棲動物活動（bioirrigation）對海岸帶生態(tài)系統(tǒng)生態(tài)過程及生物地理化學(xué)功能（如沉積有機物的再礦化）的影響。

案例2：芬蘭Turku大學(xué)利用FSO2和430μm光極氧探針，對南瓜類囊體懸浮液光合放氧進行了測量分析。

案例3：美國Woods Hole海洋學(xué)研究所，利用RF-O2非接觸式光極氧氣傳感器（sensor spot），對海洋無脊椎動物呼吸代謝進行了測量分析，以研究其固有的生物鐘與環(huán)境脅迫的關(guān)系，這些海洋無脊椎動物體重只有0.5－50mg。圖中為翼足類軟體動物在不同濃度CO2條件下的耗氧率。

案例4：澳大利亞海洋科學(xué)研究所、瑞典Gothenburg大學(xué)等組成的科學(xué)小組，利用Pyroscience的REDFLASH氧氣測量技術(shù)，對河鱸（Perca fluviatilis）呼吸代謝進行測量分析，以研究其熱耐受性和適應(yīng)性的生理機制。他們選擇波羅的海核電站附近的一個瀉湖，核電站排出的熱水進入該瀉湖，在過去30年大量魚類因為不適應(yīng)水溫升高而滅絕，但河鱸卻得以繁盛，該地成為理想的研究氣候變暖對魚類種群影響的“天然實驗室”。他們測量河鱸呼吸代謝率的同時，還測量其靜脈血液在溫度升高狀態(tài)下的氧分壓，靜脈血是河鱸心臟供氧的主要來源，高溫條件下靜脈血氧氣含量被認(rèn)為是其心臟功能的重要限制因子。

案例5：德國Ulm大學(xué)利用FSO2測量儀和50μm可伸縮式RFO2探針，對患者腦脊髓液（CSF）樣品溶解氧進行測量分析，以研究探討神經(jīng)紊亂及神經(jīng)炎等疾病的生理和診斷。

案例6：德國農(nóng)業(yè)科學(xué)與景觀研究機構(gòu)，利用FSO2測量儀和RFO2探針，對土壤氧氣進行測量，以評估不同種類蚯蚓在低氧條件下對土壤改良的效率。

案例7：西班牙Valladolid大學(xué)利用RFO2熒光光纖氧氣測量技術(shù)，監(jiān)測葡萄酒橡木桶O2吸收——對葡萄酒品質(zhì)至關(guān)重要但一直以來缺乏科學(xué)的了解。葡萄酒在橡木桶內(nèi)（3－24個月）的過程溶解氧至關(guān)重要，因為O2調(diào)節(jié)了葡萄酒整個的熟化過程。

近期部分參考文獻：

2015

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2014

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28.      Aerobic scope does not predict the performance of a tropical eurythermal fish at elevated temperatures?Norin et al., 2014, J Exp Biol Vol 217: 244-251

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