植物維生素D2(VD2)ELISA試劑盒
參考價 | ¥1260.00-¥1560.00 |
- 公司名稱 上海極威生物科技有限公司
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- 更新時間2022/12/13 14:36:50
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48T | 1260.00元 | 99 盒可售 |
96T | 1560.00元 | 99 盒可售 |
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植物維生素D2(VD2)ELISA試劑盒,英文名: Plant Vitamin D2,VD2 ELISA Kit
保質(zhì)期:六個月
品牌:國內(nèi)外ELISA試劑盒品牌
運輸:包郵(中國大陸)
植物維生素D2(VD2)ELISA試劑盒,包裝形式:盒裝(液體盒裝)
ELISA實驗操作步驟的一般常見方法
方法一)用于技術(shù)未知抗原的雙抗體夾心法:
1)包被:用0.05M PH9.牰碳酸鹽包被緩沖液將抗體稀釋至蛋白質(zhì)含量為1~10μg/ml。在每個聚苯乙烯板的反應孔中加0.1ml,4℃。次日,棄去孔內(nèi)溶液,用洗滌緩沖液洗3次,每次3分鐘。(簡稱洗滌,下同)。
2)加樣:加一定稀釋的待檢樣品0.1ml于上述已包被之反應孔中,置37℃孵育1小時。然后洗滌。(同時做空白孔,陰性對照孔及陽性對照孔)。
3)加酶標抗體:于各反應孔中,加入新鮮稀釋的酶標抗體(經(jīng)滴定后的稀釋度)0.1ml。37℃孵育0.5~1小時,洗滌。
4)加底物液顯色:于各反應孔中加入臨時配制的TMB底物溶液0.1ml,37℃10~30分鐘。
5)終止反應:于各反應孔中加入2M硫酸0.05ml。
6),結(jié)果判定:可于白色背景上,直接用肉眼觀察結(jié)果:反應孔內(nèi)顏色越深,陽性程度越強,陰性反應為無色或極淺,依據(jù)所呈顏色的深淺,以“+”、“-”號表示。也可測O·D值:在ELISA技術(shù)儀上,于450nm(若以ABTS顯色,則410nm)處,以空白對照孔調(diào)零后測各孔O·D值,若大于規(guī)定的陰性對照OD值的2.1倍,即為陽性。
方法二)用于技術(shù)未知抗體的間接法:
用包被緩沖液將已知抗原稀釋至1~10μg/ml,每孔加0.1ml,4℃。次日洗滌3次。加一定稀釋的待檢樣品(未知抗體)0.1ml于上述已包被之反應孔中,置37℃孵育1小時,洗滌。(同時做空白、陰性及陽性孔對照)于反應孔中,加入新鮮稀釋的酶標第二抗體(抗抗體)0.1ml,37℃孵育30-60分鐘,洗滌,zui后一遍用DDW洗滌。其余步驟同“雙抗體夾心法”的4、5、6。
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