植物玉米素核苷（ZR）ELISA試劑盒 ，英文名： Plant zeatin riboside,ZR ELISA Kit

國內(nèi)統(tǒng)一規(guī)格：48T/96T
保質(zhì)期：六個(gè)月
品牌：國內(nèi)外ELISA試劑盒品牌
運(yùn)輸：包郵（中國大陸）
植物玉米素核苷（ZR）ELISA試劑盒，包裝形式：盒裝（液體盒裝）
ELISA實(shí)驗(yàn)操作步驟的一般常見方法
方法一）用于技術(shù)未知抗原的雙抗體夾心法：
1）包被：用0.05M PH9.牰碳酸鹽包被緩沖液將抗體稀釋至蛋白質(zhì)含量為1～10μg／ml。在每個(gè)聚苯乙烯板的反應(yīng)孔中加0.1ml，4℃。次日，棄去孔內(nèi)溶液，用洗滌緩沖液洗3次，每次3分鐘。(簡稱洗滌，下同)。
2）加樣：加一定稀釋的待檢樣品0.1ml于上述已包被之反應(yīng)孔中，置37℃孵育1小時(shí)。然后洗滌。(同時(shí)做空白孔，陰性對照孔及陽性對照孔)。
3）加酶標(biāo)抗體：于各反應(yīng)孔中，加入新鮮稀釋的酶標(biāo)抗體(經(jīng)滴定后的稀釋度)0.1ml。37℃孵育0.5～1小時(shí)，洗滌。
4）加底物液顯色：于各反應(yīng)孔中加入臨時(shí)配制的TMB底物溶液0.1ml，37℃10～30分鐘。
5）終止反應(yīng)：于各反應(yīng)孔中加入2M硫酸0.05ml。
6），結(jié)果判定：可于白色背景上，直接用肉眼觀察結(jié)果：反應(yīng)孔內(nèi)顏色越深，陽性程度越強(qiáng)，陰性反應(yīng)為無色或極淺，依據(jù)所呈顏色的深淺，以“+”、“-”號表示。也可測O·D值：在ELISA技術(shù)儀上，于450nm(若以ABTS顯色，則410nm)處，以空白對照孔調(diào)零后測各孔O·D值，若大于規(guī)定的陰性對照OD值的2.1倍，即為陽性。
方法二）用于技術(shù)未知抗體的間接法：　　　
用包被緩沖液將已知抗原稀釋至1～10μg／ml，每孔加0.1ml，4℃。次日洗滌3次。加一定稀釋的待檢樣品(未知抗體)0.1ml于上述已包被之反應(yīng)孔中,置37℃孵育1小時(shí),洗滌。(同時(shí)做空白、陰性及陽性孔對照)于反應(yīng)孔中，加入新鮮稀釋的酶標(biāo)第二抗體(抗抗體)0.1ml，37℃孵育30-60分鐘，洗滌,zui后一遍用DDW洗滌。其余步驟同“雙抗體夾心法”的4、5、6。

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豬抗凝血酶受體(ATR)ELISA試劑盒 Porcine anti-thrombin receptor,ATR ELISA Kit Y02926 96T/48T

豬組胺(HIS)ELISA試劑盒 Porcine Histamine,HIS ELISA Kit Y02927 96T/48T

豬雌二醇(E2)ELISA試劑盒 Porcine Estradiol,E2 ELISA Kit Y02928 96T/48T

豬轉(zhuǎn)化生長因子β1(TGF-β1)ELISA試劑盒 Porcine transforming growth factor β1,TGF-β1 ELISA Kit Y02929 96T/48T

豬腫瘤壞死因子α(TNF-α)ELISA試劑盒 Porcine tumor necrosis factor α,TNF-α ELISA Kit Y02930 96T/48T