5通道96孔定量PCR儀
- 公司名稱 北京力途科技有限公司
- 品牌
- 型號
- 產(chǎn)地 韓國BIONEER
- 廠商性質(zhì) 代理商
- 更新時(shí)間 2017/7/24 6:25:58
- 訪問次數(shù) 1444
聯(lián)系我們時(shí)請說明是化工儀器網(wǎng)上看到的信息,謝謝!
產(chǎn)品詳細(xì)描述
儀器簡介
Bioneer 運(yùn)用分子生物學(xué)產(chǎn)品研發(fā)的豐富經(jīng)驗(yàn),結(jié)合的科技成果,研制出的五通道Exicycler™實(shí)時(shí)定量PCR儀。該產(chǎn)品在2004年被韓國產(chǎn)業(yè)資源部評為同類*質(zhì)量產(chǎn)品之一。儀器由熱循環(huán)反應(yīng)器和Bioneer*的實(shí)時(shí)熒光信號檢測模塊組成,利用熒光共振能量轉(zhuǎn)移原理,可以檢測兩種分子間的相互作用。具有高靈敏度、高準(zhǔn)確性和系統(tǒng)實(shí)時(shí)整合等特性,該機(jī)器性能穩(wěn)定,帶梯度功能,試劑開放。擁有CE體外診斷認(rèn)證,KFDA 醫(yī)療器械注冊證。
儀器特點(diǎn)
● 五通道,帶五個(gè)激發(fā)濾光片,五個(gè)檢測濾光片
● 光路技術(shù),使用平面光源保證均勻的照明,孔間光照強(qiáng)度差異zui小化,無需 ROX 染料校正
● 16 bit 高靈敏度 2D CCD 檢測,可一次性獲得 96 孔中全部熒光信號,孔間無時(shí)間差
● 帶梯度計(jì)算和溫度梯度功能,便于PCR 反應(yīng)條件優(yōu)化
● Touch down PCR 技術(shù)(包括Time increment、Temperature increment功能)
● 波長范圍 ( 490-670 nm ),支持常用的所有熒光染料和探針
● 動(dòng)力學(xué)線性范圍10個(gè)數(shù)量級 (101 - 1010)
● 系統(tǒng)軟件界面友好,直觀,易于學(xué)習(xí)和操作
● 試劑開放,耗材經(jīng)濟(jì)
● 認(rèn)證——CE-IVD,KFDA醫(yī)療器械注冊證
儀器優(yōu)勢
出色的2 - Dimensinal激發(fā)光源
● 采用的光路技術(shù),使用平面光源保證均勻的照明,無邊緣效應(yīng)
● 孔間光照強(qiáng)度差異zui小化
● 無需使用內(nèi)參染料校正
增強(qiáng)的光學(xué)模塊和檢測系統(tǒng)
● 使用高靈敏度2 D CCD檢測,可一次性獲得96孔中全部熒光信號
● 孔間無時(shí)間差
● 超高壓短弧光燈的明亮白光為所有的顏色提供均一的光照強(qiáng)度
● 光路模塊可將所有孔內(nèi)光損失降至zui低
● 更長的燈泡使用壽命
可靠的Ct曲線
● 在沒有使用內(nèi)參染料校正的條件下,重復(fù)的樣本間可以獲得*質(zhì)量的Ct曲線
五通道技術(shù)
● 無需為內(nèi)參染料保留一個(gè)通道,所有的五色通道都是可用的。
應(yīng)用范圍
● 基因表達(dá)定量
● 病原體檢測
● 微陣列分析
● 細(xì)菌計(jì)數(shù)/病毒滴度以及鑒定反應(yīng)產(chǎn)物
● 基于解鏈溫度的基因型檢測
實(shí)驗(yàn)結(jié)果
*的光學(xué)系統(tǒng)允許樣品的濃度信號超出系統(tǒng)的動(dòng)態(tài)、靈敏、線形的檢測范圍。
圖1.使用Accupower® GreenStar™ PCR預(yù)混合物(Bioneer貨號:K-5040)獲得的線性動(dòng)態(tài)曲線和靈敏定量結(jié)果。(A和B)將目的DNA樣品10倍系列稀釋后,分別擴(kuò)增出100 bp的產(chǎn)物。(C) C(T)值介于3.2-3.4,理論值為3.3。(D)標(biāo)準(zhǔn)曲線結(jié)果顯示相關(guān)系數(shù)超過0.999。
圖2.重復(fù)性。在不同的孔中擴(kuò)增出150 bp的相同目的DNA片段,設(shè)置8個(gè)平行實(shí)驗(yàn)。
極易操控、靈活直觀的操作軟件
Exicycler™操作系統(tǒng)以圖形界面(GUI)為基礎(chǔ),極易學(xué)習(xí)、掌握、操作,且功能強(qiáng)大。并行的結(jié)果分析功能支持在實(shí)驗(yàn)進(jìn)行中同時(shí)進(jìn)行獨(dú)立的操作和數(shù)據(jù)分析。該軟件系統(tǒng)提供持續(xù)升級,能適應(yīng)不同的操作和特征分析。
圖3.Exicycler™軟件的使用
Exicycler™實(shí)時(shí)定量PCR儀技術(shù)參數(shù)
系統(tǒng)參數(shù) | |
尺寸(mm) | 540(長) * 355(寬) * 470(高) |
重量 | 30 kg |
樣本容量 | 96 孔 / 0.2 ml管 |
樣本體積 | 20 ~ 50 μl ( 50 μl recommended ) |
電源指標(biāo) | 110 V/240 V, 50/60 Hz, 850 W |
環(huán)境溫度 | 15℃-30℃ |
環(huán)境濕度 | 20-80 |
熱學(xué)模塊參數(shù) | |
溫控方式 | 半導(dǎo)體( Peltier ) |
溫度范圍 | 4℃ - 99.9℃ |
溫度精度 | ±0.3℃ |
變溫速率 | 2.5℃/sec |
梯度功能 | 1℃-20℃ within 40℃-75℃ |
溫度/時(shí)間增量范圍 | 0.1℃-9.9℃ / 1sec-60 sec |
光學(xué)模塊參數(shù) | |
光源, | 超高壓短弧光燈( 120 W ) |
燈泡時(shí)長 | 3,000 小時(shí) |
探測器 | 16 位 2D CCD |
激發(fā)光濾片/檢測光濾片 | 各5個(gè) |
計(jì)算機(jī)參數(shù) | |
操作系統(tǒng) | Windows XP |
處理速度 | In Dual Core E2160 (1.8 GHz ) 或更高 |
內(nèi)存 | 1 GB 或更高 |
數(shù)據(jù)接口 | USB 2.0 或更高 |
顯示器分辨率 | 1280*1024或更高 |
證書
使用Exicycler™ 96發(fā)表的文章
Ref.# | Reference |
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0017 | Ultra-rapid real-time PCR for the detection of Paenibacillus larvae, the causative agent of American Foulbrood (AFB) Sang-Hoon Han, Do-Bu Lee, Dong-Woo Lee, Eul-Hwan Kim and Byoung-Su Yoon. J Invertebr Pathol. 2008 Sep;99(1):8-13. Epub 2008 May 6. More information |
0016 | CpG methylation in exon 1 of transcription factor 4 increases with age in normal gastric mucosa and is associated with gene silencing in intestinal-type gastric cancers Seung-Kyoon Kim, Hay-Ran Jang, Jeong-Hwan Kim, Mirang Kim, Seung-Moo Noh, Kyu-Sang Song, Gyeong Hoon Kang, Hee Jin Kim, Seon-Young Kim, Hyang-Sook Yoo and Yong Sung Kim.. Carcinogenesis. 2008 Aug;29(8):1623-1631. Epub 2008 Jul 16. More information |
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0014 | A viral lectin encoded in Cotesia plulae bracovirus and its immunosuppressive effect on host hemocytes Sunyoung Lee, Madanagopal Nalini, Yonggyun Kim. Comp Biochem Physiol A Mol Integr Physiol. 2008 Apr;149(4):351-361. Epub 2008 Jan 14. More information |
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